金银花中烯啶虫胺测定方法的研究  

Study on the Determination of Nitenpyram in Lonicera Japonicae Flos

作　　者：侯少岩 李嘉欣[2] 薛健[2] 王鹏思 武晓丽 袁亚莉[1] HOU Shao-yan;LI Jia-xin;XUE Jian;WANG Peng-si;WU Xiao-li;YUAN Ya-li(University of South China,College of Chemistry and Chemical Engineering,Hunan Hengyang 421000,China;Insitute of Medicinal Plant Development,Chinese Academy of Medical Science&Peking Union Medical College,Beijing 100193,China)

机构地区：[1]南华大学化学化工学院,湖南衡阳421000 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国现代中药》2018年第6期721-724,共4页Modern Chinese Medicine

基　　金：国家食品药品监督管理总局项目(ZG2016-2-03);中国医学科学院医学与健康科技创新工程经费资助(2017-12M-3-013)

摘　　要：目的:建立气相色谱法测定金银花中烯啶虫胺的方法。方法:对比不同溶剂的提取率以及固相萃取材料对金银花样品中干扰基质的净化效果,甲醇洗脱,采用DB-1701石英毛细管柱,GC-ECD测定金银花中的烯啶虫胺。结果:该方法在0.005~0.5 mg·L^(-1)范围内线性良好(r=0.999 3);添加0.02、0.1、0.2 mg·kg^(-1)时的回收率在79.14%~98.37%之间,RSD在3.66%~4.39%之间;方法的检出限为0.006 mg·kg^(-1)。结论:建立的检测方法灵敏度高,准确度高,线性好,符合农药残留分析要求,可用于金银花中烯啶虫胺的测定。Object:To establish a gas chromatographic method for the determination of nitenpyram in Lonicerae Japonicae Flos.Methods:The extraction rate of different solvents and the purification effect of solid phase extraction material on the interference matrix in the sample were eluted with methanol.The DB-1701 quartz capillary column and GC-ECD were used to determine nitenpyram in Lonicerae Japonicae Flos.Results:The method showed a good linearity(r=0.999 3)in the range of 0.005~0.5 mg·L-1.The recoveries were 79.14%~98.37%with addition of 0.05,0.1 and 0.2 mg·kg-1 respectively,and with RSDs of 3.66%~4.39%.The detection limit of the method is 0.006 mg·kg-1.Conclusion:The established detection method has the advantages of high sensitivity,high accuracy and good linearity,in line with the requirements of pesticide residue analysis,and can be used for the determination of nitenpyram residues in Lonicerae Japonicae Flos.

关 键 词：金银花 烯啶虫胺 气相色谱法 残留测定 

分 类 号：S481.8[农业科学—农药学] S567.79[农业科学—植物保护]