迟缓爱德华菌鞭毛蛋白FliC原核表达及免疫试验  

Prokaryotic Expression and Immune Test of FliC Protein of Edwardsiella tarda

作　　者：张志强[1] 杨楠 李永慧 王洪彬 吴同垒[1] 康元环[3] 莘余 史秋梅[1] 高桂生[1] 王春凤[3] ZHANG Zhi-qiang;YANG Nan;LI Yong-hui;WANG Hong-bin;WU Tong-lei;KANG Yuan-huan;XIN Yu;SHI Qiu-mei;GAO Gui-sheng;WANG Chun-feng(Hebei Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science&Technology,Qinhuangdao,Hebei, 066004,China;The Second Hospital of Qinhuangdao,Changli,Hebei,066600;College of Animal Science and Technology, Jilin Agriculture University,Changchun,Jilin,130118,China)

机构地区：[1]河北省预防兽医学重点实验室/河北科技师范学院,河北秦皇岛066004 [2]秦皇岛第二医院,河北昌黎066600 [3]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《动物医学进展》2018年第7期23-27,共5页Progress In Veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0501000);中国博士后科学基金(2017M611935);河北省科技厅奖励性后补助项目(15926620H);秦皇岛市科技局科技支撑计划(201401A067;201402B043);河北科技师范学院博士启动基金(2015YB002)

摘　　要：为了解迟缓爱德华菌鞭毛蛋白FliC的免疫特性,从迟缓爱德华菌基因组中克隆出鞭毛基因fliC,并将其构建到原核表达载体上,用原核表达的方法获得大量重组蛋白,将蛋白纯化后,通过动物模型确定该蛋白的免疫特性。结果表明,迟缓爱德华菌鞭毛蛋白FliC具有较强的免疫原性,对免疫动物应对强毒株感染具有一定保护效果。将FliC与牛血清白蛋白(BSA)混合免疫小鼠能够刺激机体产生较高水平抗BSA抗体,说明其佐剂特性。研究表明,迟缓爱德华菌鞭毛蛋白FliC具有优良的免疫原性和佐剂特性,是迟缓爱德华菌的保护性抗原,具有潜在的应用价值。To investigate the immunological characters of flagellar protein FliC,in this study,fliC gene of Edwardsiella tarda was amplificated by PCR and cloned into pET-32a vector for for prokaryotic expression and purification,and abundant purified His-FliC was obtained.The recombinant FliC were then performed as immunogen for further immunological characters analysis.The data determined that,the FliC protein is able to sitmulate strong immune response and a certain specific protection to E.tarda infection.And the mice,received immunization combined FliC with BSA,displayed considerable anti-BSA serum antibody level,demonstrating the adjuvant characteristics of FliC.All these work showed that the flagellar protein FliC of E.tarda possess excellent immunogenicity and adjuvant characteristics,and may be a good protective antigen of E.tarda for vaccine development.

关 键 词：迟缓爱德华菌 鞭毛蛋白 FLIC 佐剂 免疫保护力 

分 类 号：S852.612[农业科学—基础兽医学]