菲律宾蛤仔中一株枯草芽胞杆菌的分离鉴定及生物学特征研究  

Isolation, identification and biological characteristics of a Bacillus subtilis from Ruditapes philippinarum

作　　者：毛晴 陈丰 梁建 刘军锋 吕爱军 胡秀彩 郭永军 邢克智 孙敬锋 MAO Qing;CHEN Feng;LIANG Jian;LIU Jun-feng;LüAi-jun;HU Xiu-cai;GUO Yong-jun;XING Ke-zhi;SUN Jing-feng(Tianjin Key Lab of Aqua-Ecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;Tianjin Yushengtang Biotechnology Co.Ltd.,Tianjin 300404,China)

机构地区：[1]天津农学院水产学院天津市水产生态及养殖重点实验室,天津300384 [2]天津市渔生堂生物技术有限公司,天津300404

出　　处：《天津农学院学报》2018年第2期42-46,50,共6页Journal of Tianjin Agricultural University

基　　金：国家现代农业产业技术体系项目(CARS-48);天津市水产产业技术体系创新团队项目(ITTFRS2017009;ITTFRS2017013);天津市北辰区科技创新专项项目(KJCX-XDZ-KTP-2016-005)

摘　　要：从健康菲律宾蛤仔(Ruditapes philippinarum)肝胰脏中分离纯化出一株细菌,命名为G6,通过形态学观察、生理生化特性、16S r DNA序列测定、系统发育分析等方法鉴定为枯草芽孢杆菌(Bacillus subtilis)。然后对其生长曲线、耐高温、耐酸性、耐胆盐等生物学特征进行研究。结果表明,G6菌株生长期较长,在26~34 h进入稳定期,具有较强的耐高温、耐酸性、耐胆盐等特性。本研究为枯草芽孢杆菌G6菌株作为水产动物益生菌菌株的开发应用奠定了基础。A bacterium strain was isolated and purified from the hepatopancreas of healthy Ruditapes philippinarum and named G6.This bacterium strain was identified as Bacillus subtilis according to the results of morphological observation,physiological and biochemical characteristics,16S rDNA sequence,and phylogenetic analysis.Its biological characteristics were studied,including growth curve,resistance to high temperature,acid,and bile salt.Results showed that the strain G6 had a long growth period and a stationary phase from 26 to 34 h and was resistant to high temperature,acid,and bile salt.This report provided a scientific base for application of G6 as the probiotic for aquatic animals.

关 键 词：菲律宾蛤仔 枯草芽孢杆菌 分离 鉴定 生长特性 耐受性 

分 类 号：S917.4[农业科学—水产科学]