四倍体果桑嘉陵30号再生植株的研究  

Study on Plant Regeneration of the Tetraploid Fruit Mulberry Cultivar Jialing 30

作　　者：植爽[1] 任艳红[1] 唐星 徐凤翔 王茜龄[1] 赵爱春[1] ZHI Shuang;REN Yan-hong;TANG Xing;XU Feng-xiang;WANG Xi-ling;ZHAO Ai-chun(College of Biotechnology,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学生物技术学院,重庆400715

出　　处：《蚕学通讯》2018年第1期1-9,共9页Newsletter of Sericultural Science

基　　金：国家蚕桑产业技术体系建设专项(CARS-18-ZJ0201);中央高校基本科研业务费(XDJK2017D116);重庆市留学人员创业创新支持计划(CX2017130)

摘　　要：以四倍体果桑品种嘉陵30号(Morus atropupurea Roxb.)的春芽和冬芽为外植体,建立了嘉陵30号的高频再生体系。结果表明,冬芽比春芽更适合作外植体,低温处理后再用赤霉素处理对解除冬芽休眠效果最好;0.1%HgCl_2消毒15min且在培养基中添加300mg/L噻孢霉素钠盐(Cef,Cefotaxime Sodium Salt)能有效控制污染;培养基MS+3.0mg/L 6-BA+0.2mg/L NAA适用于果桑的初代和继代培养。嘉陵30号通过直接器官发生途径再生,使用不定芽诱导丛生芽此种增殖方式效果更佳,增值系数高达7.04,且苗粗壮,生长旺盛,最适诱导培养基为1/2MS+0.6mg/L IAA,生根率可达87.5%,根系粗壮。A high frequency regeneration system for the tetraploid fruit mulberry(Morus atropupurea Roxb.)cultivar Jialing 30 was established,using its spring buds and winter buds as the explants.The results showed that used as explants,winter buds were more suitable than spring buds,and that low temperature treatment in combination with subsequent GA 3 treatment gave the best results of breaking dormancy of winter buds.Contamination was effectively controlled by means of disinfecting with 0.1%HgCl 2 for 15 min and adding 300 mg/L cefotaxime sodium salt into the medium.MS medium supplemented with 3.0 mg/L 6 BA and 0.2 mg/L NAA was suitable for the primary culture and subculture of Jialing 30.The pathway of regeneration for Jialing 30 was direct organogenesis in vitro.Induction of cluster buds from adventitious buds was a more desirable approach for the multiplication of Jialing 30,its multiplication factor being 7.03,and the plants regenerated being healthy and vigorous.The best rooting medium for Jialing 30 was 1/2MS medium supplemented with 0.2 mg/L IAA,which gave a rooting percentage of 87.5%,with strong and vigorous roots.

关 键 词：果桑 嘉陵30号 组织培养 再生 

分 类 号：S888[农业科学—特种经济动物饲养]