桑树SSR实验技术要点分析  

Analysis of the Key Points in SSR Experiment Technologies for Mulberry

作　　者：刘玲 傅晓 柯皓天 吕银[2] LIU Ling;FU Xiao;KE Hao-tian;LU Yin(Silk Engineering Technology Research Center of Sichuan Province,Chengdu 610031,China;Silk Science Research Institute of Sichuan Province,Chengdu 610031,China)

机构地区：[1]四川省丝绸工程技术研究中心,四川成都610031 [2]四川省丝绸科学研究院,四川成都610031

出　　处：《蚕学通讯》2018年第1期17-20,共4页Newsletter of Sericultural Science

基　　金：四川省科技计划项目(2017JY0277)

摘　　要：本文分析了桑树SSR的实验过程:基因组DNA提取、引物筛选、PCR反应体系优化、引物退火温度选择、凝胶配制、电泳和凝胶染色、显色,并指出了这些步骤中需要注意的问题,为SSR技术在桑树研究中的应用提供基础。In order to provide a foundation for the application of SSR(Simple Sequence Repeat)technology in mulberry research,this paper analyzes the experimental process of SSR used in mulberry:genomic DNA extraction,primer screening,optimization of SSR PCR reaction system,primer annealing temperature selection,gel preparation,electrophoresis,gel staining and color development,and points out the issues that should be paid attention to in these steps.

关 键 词：桑树 SSR 实验技术要点 

分 类 号：S888[农业科学—特种经济动物饲养]