饲料中添加谷胱甘肽对黄颡鱼幼鱼组织谷胱甘肽含量、免疫及抗氧化性能的影响  

EFFECTS OF DIETARY SUPPLEMENTATION WITH GLUTATHIONE ON TISSUE GSH LEVEL, IMMUNITY, AND ANTIOXIDANT CAPACITY OF JUVENILE YELLOW CATFISH(PELTEOBAGURS FULVIDRACO)

作　　者：周艳玲 孙育平[1,3,4] 黄燕华 王国霞 陈冰 王卫民 曹俊明[1,3,4] 易昌金 ZHOU Yan-Ling;SUN Yu-Ping;HUANG Yan-Hua;WANG Guo-Xia;CHEN Bin;WANG Wei-Min;CAO Jun-Ming;YI Chang-Jin(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture,Guangzhou 510640,China;Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院动物科学研究所,广州510640 [2]华中农业大学水产学院,武汉430070 [3]农业部华南动物营养与饲料重点实验室,广州510640 [4]广东省畜禽育种与营养研究重点实验室,广州510640

出　　处：《水生生物学报》2018年第4期728-735,共8页Acta Hydrobiologica Sinica

基　　金：广东省现代农业科技创新联盟建设(2017LM082和2017LM083);2017年广东省渔业厅海洋和渔业发展专项资金(科技攻关与研发)资助~~

摘　　要：研究旨在探讨饲料中添加还原型谷胱甘肽(Glutathione,GSH)对黄颡鱼幼鱼(Pelteobagrus fulvidraco)组织谷胱甘肽含量、免疫及抗氧化性能的影响。选用初始体重为(1.32±0.01)g的黄颡鱼800尾,随机分为5组,每组4个重复,每个重复40尾鱼,分别投喂基础饲料和添加100、300、500和700 mg/kg GSH的试验饲料,饲养56d后采样分析,并采用氯化铵进行96h氨氮应激试验。结果表明:除100 mg/kg组外,饲料中添加GSH显著提高黄颡鱼肝脏、血清GSH含量(P<0.05),当GSH添加量≥300 mg/kg时,肝脏和血清GSH含量均呈现稳定状态。随着饲料中谷胱甘肽水平的增加,血清免疫和肝脏抗氧化指标均呈现先升高后降低的趋势,其中300和500 mg/kg组溶菌酶与碱性磷酸酶活性、300 mg/kg组免疫球蛋白M与补体4含量、500 mg/kg组酸性磷酸酶活性与对照组相比显著升高(P<0.05)。与对照组和700 mg/kg组相比,300 mg/kg组肝脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化酶活性和总抗氧化能力与血清超氧化物歧化酶、谷胱甘肽过氧化酶活性均显著高升高(P<0.05);且300 mg/kg组血清丙二醛含量显著降低(P<0.05)。氨氮应激96h时,与对照组相比,300 mg/kg组肝脏和血清超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化酶活性力均显著升高(P<0.05),且300 mg/kg组血清丙二醛含量显著降低(P<0.05)。由此可见,饲料中添加谷胱甘肽能提高黄颡鱼幼鱼组织谷胱甘肽含量、免疫及抗氧化性能,其中以300—500 mg/kg为宜。To assess the effects of dietary supplementation with glutathione(GSH)on tissue GSH levels,immunity,and antioxidant capacity of juvenile yellow catfish(Pelteobagrus fulvidraco),a total of 800 yellow catfish with a mean body weight of(1.32±0.01)g were considered and randomly divided into 5 groups with 4 replicates per each group and 40 fish per each replicate as well.During 56d,the fish were fed by a basal diet and four test diets,which supplemented with 100,300,500,and 700 mg/kg of reduced GSH,respectively.The results indicated that,except for 100 mg/kg group,supplementation with GSH significantly increased the GSH content in liver and serum of yellow catfish(P<0.05).When GSH level was 300 mg/kg or higher,GSH content in liver and serum was in a stable state.With the increase of glutathione level,the immunity and antioxidant indices were initially increased,and then decreased.The lysozyme and alkaline phosphatase activities in 300 and 500 mg/kg groups,immunoglobulin M(IgM)and complement component 4(C4)contents in 300 mg/kg group,and acid phosphatase activity in 500 mg/kg group markedly increased,compared with the control group(P<0.05).There were no significant differences(P>0.05)in complement component 3(C3)and nitric oxide contents among all groups.Compared with the control and 700 mg/kg groups,the liver superoxide dismutase,catalase and glutathione peroxidase activities,total antioxidant capacity and the serum superoxide dismutase,as well as glutathione peroxidase activities in 300 mg/kg group were significantly higher(P<0.05),while the serum malondialdehyde content in that group was considerably lower(P<0.05).Under ammonia-nitrogen stress for 96h,compared with the control group,the liver and serum superoxide dismutase,catalase,and glutathione peroxidase activities in 300 mg/kg group were significantly greater(P<0.05),however,the serum malondialdehyde content in the mentioned group was significantly lower(P<0.05).In conclusion,dietary supplementation with GSH could enhance the tissue GSH level,immunity and anti

关 键 词：谷胱甘肽 黄颡鱼 GSH含量 免疫 抗氧化 氨氮应激 

分 类 号：S965.1[农业科学—水产养殖]