生长素吲哚乙酸对铜绿微囊藻生理生化及产毒特性的影响  

EFFECTS OF IAA ON THE PHYSIOLOGICAL AND TOXIN-PRODUCING CHARACTERISTICS OF MICROCYSTIS AERUGINOSA

作　　者：卿纯 张卉灵 林毅青[1] 李俊 胡婷 邵继海[1] QING Chun;ZHANG Hui-Ling;LIN Yi-Qing;LI Jun;HU Ting;SHAO Ji-Hai(Hunan Agricultural University,Changsha 410128,China;Orient Science and Technology College of Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学资源环境学院,长沙410128 [2]湖南农业大学东方科技学院,长沙410128

出　　处：《水生生物学报》2018年第4期832-838,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31470511)资助~~

摘　　要：为了探究生长素吲哚乙酸(IAA)对产毒铜绿微囊藻(Microcystis aeruginosa)的影响,从生长、光合色素含量、叶绿素光诱导荧光特征、脂质氧化和微囊藻毒素合成特性等方面,研究了IAA对M.aeruginosa CHAB6301生理生化及产毒的影响。结果表明,在低浓度IAA(0.04和0.2 mg/L)条件下,铜绿微囊藻生长、叶绿素含量、光合系统(PSⅡ)电子传递效率及藻毒素含量均无明显变化,藻蓝蛋白、别藻蓝蛋白和丙二醛(MDA)含量均低于对照。高浓度IAA(0.04和0.2 mg/L)能够促进细胞生长,提高叶绿素含量,但是抑制藻蓝蛋白和别藻蓝蛋白含量,降低膜脂过氧化程度和细胞内藻毒素合成。综合各指标测定结果,低浓度IAA对M.aeruginosa CHAB6301生长和光合作用影响不明显,而高浓度IAA可促进藻细胞生长和光合作用,增加微囊藻水华形成几率。In order to investigate the effects of indole-3-acetic acid(IAA)on physiological characteristics of Microcystis aeruginosa(M.aeruginosa),the growth,photosynthetic pigment content,chlorophyll fluorescence,lipid oxidation,and microcystin biosynthesis characteristics were studied.The achieved results showed that there was no significant variation in cell density,chlorophyll content,chlorophyll fluorescence characteristics,and microcystin contents at low concentrations of IAA(e.g.,0.04 and 0.2 mg/L).However the contents of phycocyanin,allophycocyanin,and malondialdehyde(MDA)decreased in comparison with the control group.High concentrations of IAA(0.04 and 0.2 mg/L)accelerate the algal growth and increased chlorophyll content,while that inhibited the contents of phycocyanin and allophycocyanin and reduced the peroxidation of membrane lipid peroxidation as well as synthetization of intracellular algal toxins.The findings also demonstrated that low concentrations of IAA have no clear influence on the growth and photosynthesis of M.aeruginosa CHAB-6301.However,high concentrations of IAA can promote the growth and photosynthesis of this alga,which may increase the possibility of formation of Microcystis-based water blooms.

关 键 词：吲哚乙酸 铜绿微囊藻 光合色素 叶绿素荧光 微囊藻毒素 

分 类 号：Q938.8[生物学—微生物学]