19个X-STR基因座在中国3个民族的遗传学调查及法医学应用  

Genetic polymorphisms of 19 X-STR loci for forensic application in China's three ethnicities

作　　者：刘亚举 龙飞[2] 李瑾 岳俊涛 石美森 LIU Ya-ju;LONG Fei;LI Jin;YUE Jun-tao;SHI Mei-sen(Criminal Science and Technology Institute,Public Security Bureau of Xuchang in Henan Province,Xuchang 461000;Forensic Team,Public Security Bureau of Zunyi City in Guizhou Province,Zunyi 563000;Key Laboratory of Evidence Science of Ministry of Education,China University of Political Science and Law,Beijing 100192,China)

机构地区：[1]河南省许昌市公安局刑事科学技术研究所,河南许昌461000 [2]贵州省遵义市公安局刑侦支队,贵州遵义563000 [3]中国政法大学证据科学教育部重点实验室,北京100192

出　　处：《基础医学与临床》2018年第7期913-921,共9页Basic and Clinical Medicine

基　　金：国家自然科学基金(81671874)

摘　　要：目的用Microreader^(TM) 19X ID System试剂盒调查19个X染色体短串联重复序列(X-STR)基因座在中国汉族、仡佬族、苗族人群中的遗传学数据,评估其法医学应用价值。方法采集汉(308名)、仡佬(398名)和苗(323名)族无关个体外周血样DNA进行PCR扩增,3500XL遗传分析仪电泳分析,Gene Mapper ID-X分析等位基因片段大小。统计分析19个X-STR基因座的等位基因频率和群体遗传学参数,对3个民族之间的分布差异进行分析,并与其他地区已有人群数据进行比较。结果经Bonferroni修正,19个X-STR基因座基因型分布在3个民族群体中符合Hardy-Weinberg平衡定律;基因座之间不存在连锁不平衡现象。这19个X-STR基因座在女性群体的累积个人识别率均大于0.999 999 999 99,在男性群体的累积个人识别率均大于0.999 999 999 94,在三联体中的累积非父排除率均大于0.999 999 999 36,在二联体中的累积非父排除率均大于0.999 999 52。通过Arlequin v3.5软件计算P值,不同人群间X-STR基因座的频率分布差异呈显著性。结论这19个X-STR基因座在中国汉族、仡佬族和苗族人群中具有较高的多态性,能达到较高的个体识别能力,可以作为现有STR检验体系的补充。Objective To investigate the genetic data of the 19 X-STR loci in three ethnicities of China(Han,Gelao,Miao)and to evaluate the application in forensic science.Methods The DNA samples of unrelated individ-ual in Han(n=308),Gelao(n=398),Miao(n=323)ethnicities were amplified using Microreader TM 19X ID System kit,and the PCR products were analyzed by electrophoresis through 3500XL genetic analyzer.The fragment sizes of alleles were taken subsequently by GeneMapper ID-X.Allele frequencies and national genetics parameters of the 19 X-STR were analyzed by statistics.The allele frequencies were compared among the three nationalities and were compared with available data of other Han ethnicities from different regions.Results After the Bonferroni correction at a 95%significance level,no significant departures from the Hardy-Weinberg equilibrium was observed.Linkage disequilibrium test showed no significant allelic association between all 19 X-STR loci after Bonferroni s correction.The cumulative discrimination power in females and in males were greater than 0.999 999 999 99 and 0.999 999 999 94,respectively.The combined power of exclusion in trios and in duos were greater than 0.999 999 999 36 and 0.999 999 52,respectively.The p values,calculated throuth Arlequin v3.5 software,there were significantly different as detected at loci of X-STR among the different nationalities.Conclusions This panel of X-STR is highly polymorphic in China s three ethnicities and can be served as a supplementary to the current STR system for individual identification.

关 键 词：X染色体短串联重复序列 遗传多态现象 汉族 仡佬族 苗族 

分 类 号：R89[医药卫生—法医学]