硼对大鼠脾脏淋巴细胞的毒性作用及增殖和凋亡的影响  

Effects of Boron on the Cytotoxicity,Proliferation and Apoptosis of Spleen Lymphocytes in Rats

作　　者：裴亚琼 张倩[1] 胡倩倩[1] 李升和[1] 金光明[1] 靳二辉[1] PEI Yaqiong;ZHANG Qian;HU Qianqian;LI Shenghe;JIN Guangming ;JIN Erhui(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2018年第2期7-13,共7页Journal of Anhui Science and Technology University

基　　金：国家自然科学基金(31402154;31502137;31672502);安徽省教育厅重点项目(KJ2017A505);安徽科技学院科研基金重点项目(ZRC2016476);安徽科技学院稳定人才基金项目(ZRC2013354);安徽科技学院重点学科建设项目(AKZDXK2015A04);安徽省第七批"115"产业创新团队项目(皖人才[2014]4号)

摘　　要：目的:研究不同浓度硼对大鼠脾脏淋巴细胞毒性作用,以及对其增殖和凋亡的影响。方法:选用2月龄清洁级Sprague Dawley(SD)雄性大鼠,无菌分离脾脏淋巴细胞,将其分为对照组和试验Ⅰ~组,分别在淋巴细胞中添加0、0.1、0.2、0.4、0.8、1、2、4、8、10、20、40、80、100mmol/L的硼酸,处理24h后,MTT和Cell-Counting Kit-8法检测硼对淋巴细胞的毒性作用,流式细胞仪检测淋巴细胞增殖和凋亡情况。结果:细胞毒性试验表明,与对照组相比,试验Ⅲ组淋巴细胞抑制率显著降低(P<0.05),增殖率显著升高(P<0.05),而试验Ⅹ~I组淋巴细胞抑制率极显著的升高(P<0.01),增殖率极显著的降低(P<0.01);流式细胞仪检测表明,试验Ⅲ组淋巴细胞增殖率较对照组极显著升高(P<0.01),凋亡率显著降低(P<0.05),而试验Ⅺ和Ⅻ组凋亡率均极显著升高(P<0.01),增殖率均极显著降低(P<0.01)。结论:添加0.4mmol/L的硼酸对淋巴细胞没有毒性作用,可以显著刺激淋巴细胞的增殖,抑制其凋亡,当添加量达到20mmol/L时,则对淋巴细胞产生明显的毒性作用,提高淋巴细胞的凋亡率。Objective:To study the effects of different concentrations of boron on the toxicity,proliferation and apoptosis of spleen lymphocytes in rats.Methods:20 clean grade Sprague Dawley(SD)male rats(2 months)were chosen and splenic lymphocytes were isolated aseptically.They were divided into control group and experimental group(I-XIII),which added in the lymphocytes respectively 0,0.1,0.2,0.4,0.8,1,2,4,8,10,20,40,80 and 100 mmol/L boric acid for 24 h,determined by MTT,and Cell Counting Kit-8 method are used to detect boron cytotoxic effects,and flow cytometry to detect lymphocyte proliferation and apoptosis.Results:Cytotoxicity test results can be seen that,compared with the control group,the inhibition rate was significantly decreased in the experimental group III(P<0.05),the proliferation rate was significantly increased(P<0.05),but the inhibition rate of experimental group X-XIII was significantly increased(P<0.01)with the proliferation rate was significantly decreased(P<0.01).Flow cytometry results can be seen that,compared with the control group,the proliferation rate of experimental group III was significantly increased(P<0.01),and the apoptosis rate was significant decreased(P<0.05),and the proliferation rate of experimental group XI and XII were significantly decreased(P<0.01)with the apoptosis rate were significantly increased(P<0.01).Conclusion:Adding 0.4 mmol/L of boric acid is no cytotoxic effect on lymphocytes,It can significantly stimulate lymphocyte proliferation and decrease the inhibition rate of lymphocytes.When adding 20 mmol/L of boric acid,it has significantly cytotoxic effects on lymphocytes and promoting the apoptosis of lymphocytes.

关 键 词：硼 大鼠 脾脏淋巴细胞 细胞毒性 增殖 凋亡 

分 类 号：Q952.5[生物学—动物学]