补喂5-羟色氨酸、瘤胃保护性5-羟色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响  

Effects of Diet Supplemented with 5-Hydroxytryptophan,Rumen Protected 5-Hydroxytryptophan on Plasma 5-Hydroxytryptophan and Melatonin Contents of Sheep

作　　者：赵芳[1] 高超[1] 王根 赵国栋[1] 李晓斌[1] 马晨[1] 杨开伦[1] ZHAO Fang;GAO Chao;WANG Gen;ZHAO Guodong;LI Xiaobin;MA Chen;YANG Kailun(Xinjiang Key Laboratory of Meat&Milk Production Herbivore Nutrition,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,新疆肉乳用草食动物营养重点实验室,乌鲁木齐830052

出　　处：《动物营养学报》2018年第6期2143-2154,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31760681)。

摘　　要：本试验旨在研究补喂5-羟色氨酸、瘤胃保护性5-羟色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响,探究通过5-羟色氨酸调节绵羊机体中褪黑素分泌模式及含量的可能性。试验选取1岁、平均体重为(55.78±3.24)kg的陶赛特母羊18只,按体重分为3组,每组6只,分别为对照组和试验Ⅰ组、试验Ⅱ组,每天每只羊的粉状精料饲喂量为体重的1%,玉米青贮饲喂量为0.6 kg,混合干草自由采食,在此基础上,试验Ⅰ组、试验Ⅱ组羊只分别补喂5-羟色氨酸50 mg/kg BW、瘤胃保护性5-羟色氨酸111 mg/kg BW,进行15 d的饲养试验。结果表明:在早上、下午饲喂后试验组血浆中5-羟色氨酸含量均高于对照组,且在下午饲喂后2个试验组血浆中5-羟色氨酸含量呈波动性上升,但试验Ⅰ组与试验Ⅱ组之间差异不显著(P>0.05)。在早上饲喂后1.5 h,各组血浆中色氨酸含量均呈增加趋势;在下午饲喂后1.5~6.0 h,各组血浆中色氨酸含量呈下降趋势。在白天时,各组血浆中5-羟色胺含量差异不显著(P>0.05);在下午饲喂后1.5~6.0 h,各组血浆中5-羟色胺含量呈波动性上升。在早上饲喂后3.0~9.0 h,试验Ⅰ组与试验Ⅱ组血浆中褪黑素含量均高于对照组,其中在早上饲喂后6.0 h试验Ⅰ组和试验Ⅱ组与对照组的差异均达到了极显著水平(P<0.01)。在下午饲喂后1.5~9.0 h,各组血浆中褪黑素含量均呈波动性上升,且在下午饲喂后1.5、3.0、6.0 h试验Ⅰ组和试验Ⅱ组极显著高于对照组(P<0.01),在下午饲喂后9.0 h试验Ⅱ组显著高于对照组(P<0.05)。由此可知,补喂5-羟色氨酸(50 mg/kg BW)或瘤胃保护性5-羟色氨酸(111 mg/kg BW)均可显著提高绵羊血浆中5-羟色氨酸含量,改变白天机体中褪黑素分泌模式及含量,且瘤胃保护性5-羟色氨酸的作用更有效,但对血浆中色氨酸、5-羟色胺含量均无显著影响。This experiment aimed to study the effects of diet supplemented with 5-hydroxytryptophan,rumen protected 5-hydroxytryptophan on plasma 5-hydroxytryptophan and melatonin contents of sheep,and to explore the possibility of regulating sheep melatonin secretion mode and content by 5-hydroxytryptophan.Eighteen 1-year-old Dorset sheep with the average body weight of(55.78±3.24)kg were divided into 3 groups according to the body weight,being control group,trial groupⅠand trial groupⅡ.Each sheep in all group was fed with 10%of body weight powder concentrate,0.6 kg corn silage every day,and had free continuous access to mixed hay,additionally,each sheep in trail groupⅠand trial groupⅡwas fed with 5-hydroxytryptophan(50 mg/kg BW)and rumen protected 5-hydroxytryptophan(111 mg/kg BW),respectively.The supplementary feeding experiment lasted for 15 days.The results showed that plasma 5-hydroxytryptophan content in trial groups were higher than that in control group after feeding in the morning and afternoon,and 5-hydroxytryptophan content in trial groups showed a fluctuating increasing trend after feeding in the afternoon,but the differ ence between two trial groups was not significant(P>0.05).Plasma tryptophan content in all groups was in ence between two trial groups was not significant(P>0.05).Plasma tryptophan content in all groups was in ence between two trial groups was not significant(P>0.05).Plasma tryptophan content in all groups was in creased at 1.5 h after feeding in the morning,but it was decreased at 1.5 to 6.0 h after feeding in the afternoon.During the daytime,there was no significant difference in plasma 5-hydroxytryptamine content among all groups(P>0.05).The content of 5-hydroxytryptamine in each group was volatility increased at 1.5 to 6.0 h after feeding in the afternoon.Plasma melatonin content in trail groupⅠand trial groupⅡwas higher than that in control group at 3.0 to 9.0 h after feeding in the morning.Among them,the differences between two trial groups and control groups at 6.0 h after

关 键 词：绵羊 褪黑素 5-羟色氨酸 5-羟色胺 

分 类 号：S816[农业科学—饲料科学]