三种血酮仪对奶牛酮病诊断效果的比较  

Comparison of Diagnosis effectiveness among three kinds of Blood Ketone Meters on Ketosis in dairy Cattles

作　　者：刘建波 田琳琳 李兴华 白云龙[4] 李鑫 夏成[4] 徐闯[4] 张洪友[4] Liu Jianbo;Tian Linlin;Li Xinghua;Bai Yunlong;Li Xin;Xia Cheng;Xu Chuang;Zhang Hongyou(Mudanjiang management station of the slaughtered livestock and poultry,HeiLongJiang Mudanjiang 157011;Daqing management station of the slaughtered livestock and poultry,Mudanjiang,HeiLongJiang Daqing 163319;Suihua Yuda animal husbandry Co.,Ltd.,HeiLongJiang Suihua 157000;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,HeiLongJiang Daqing 163319;WuhanKelibo Animal husbandry technology Co.,Ltd.,Hubei Wuhan 430000)

机构地区：[1]黑龙江省牡丹江市畜禽屠宰管理所,黑龙江牡丹江157011 [2]黑龙江省大庆市畜禽屠宰管理所,黑龙江大庆163319 [3]绥化市裕达牧业有限公司,黑龙江绥化157000 [4]黑龙江八一农垦大学,黑龙江大庆163319 [5]武汉科立博牧业科技有限公司,湖北武汉430000

出　　处：《现代畜牧兽医》2018年第6期26-30,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家自然基金面上项目(30972235);国家自然科学基金面上项目(31372488);黑龙江省应用技术研究与开发计划重大项目(GA16B202);黑龙江省农垦总局"十三五"重点科技攻关项目(HNK135-04-03)

摘　　要：本试验应用3种血酮仪检测奶牛酮病,经综合指标分析,为奶牛场血酮仪的选择提供依据。在黑龙江某集约化奶牛场,随机选取3~6胎次,产后5~21 d泌乳奶牛29头,经尾静脉采血分离血清。应用3种血酮仪(雅培、BHBCheck、怡成)与生化仪对比检测奶牛血清酮体。结果显示:雅培,敏感度87.5%,特异度100%,准确率93.1%,Youden指数87.5%;怡成:敏感度75%,特异度100%,准确率86.2%,Youden指数75%;BHBCheck:敏感度93.8%,特异度100%,准确率96.5%,Youden指数93.8%。并且,ROC曲线下面积,雅培为0.938,怡成为0.875,BHBCheck为0.969。在价格方面,雅培30元/例,BHBCheck 23元/例,怡成15元/例。结论:综合评价三种血酮仪,BHBCheck性价比最优。In this study,three kinds of common blood ketone meters were used to diagnose ketosis in dairy cows,accompanied by comprehensive index analysis.Its aim is to provide a basis on choice of blood ketone meters in dairy farms in future.29 dairy cows with 3~6 parity were randomly selected at 7~21 days postpartum in this experiment.Their blood samples collected by tail vein were centrifuged to obtain serum,and three kinds of blood ketone meters(Yapei,BHBCheck and Yicheng)were compared with biochemical analysis meter to measureβ-hydroxybutyric acid concentraion from serum samples of all experimental cows.The results showed that Yapei’s sensitivity is 87.5%,specificity is 100%,accuracy is 93.1%,Youden Index is 87.5%,area under the curve(AUC)of ROC is 0.938,Yicheng’s sensitivity is 75%,specificity is 100%,accuracy is 86.2%,Youden Index is75%,AUC of ROC is 0.875;BHBCheck’s sensitivity is 93.8%,specificity is 100%,accuracy is 93.8%,AUC of ROC is 0.969.In diagnosis cost,Yapei is 30RMB per cow,BHBCheck is 23RMB,Yicheng is 15RMB.Conclusion obtained that the BHBCheck is best in comprehensive evaluation.

关 键 词：血酮仪 奶牛酮病 综合评估 

分 类 号：S858.23[农业科学—临床兽医学]