ELISA法和胶体金试纸条法在乙肝表面抗原检测中的应用研究  

Application of ELISA and Colloidal Gold Strip in the Detection of Hepatitis B Surface Antigen

作　　者：邬剑[1] 李慧玲[2] WU Jian;LI Hui-ling(Department of Clinical Laboratory,Jiamusi Maternal and Child Health Hospital,Jiamusi 154002,Heilongjiang,China;Department of Clinical Laboratory,First Affiliated Hospital of Jiamusi University,Jiamusi 154002,Heilongjiang,China)

机构地区：[1]佳木斯市妇幼保健院检验科,黑龙江佳木斯154002 [2]佳木斯大学附属第一医院检验科,黑龙江佳木斯154002

出　　处：《医学信息》2018年第9期155-156,共2页Journal of Medical Information

摘　　要：目的研究ELISA法和胶体金试纸条法(GICA)在乙肝表面抗原检测中的准确度和特异性,分析方法的应用价值。方法选取本院妇幼保健院检验科在2016年5月~2017年12月收集的共计460例血样标本,分别运用ELISA法及GICA法进行检测,比较检测结果。结果 ELISA法乙肝表面抗原阳性率为47.61%,GICA法阳性率为41.96%,差异有统计学意义(P<0.05)。ELISA法及GICA法在对乙型病毒性肝炎表面抗原检测结果上具有密切的相关性(P<0.05)。ELISA法灵敏度和特异度均高于GICA法(P<0.05)。结论 ELISA法在阳性率、特异度及灵敏度等指标上均较GICA法高,但由于GICA法器材更为小巧、简洁直观,且无需大型设备辅助,较为广泛地应用于普查及急诊前检查中。Objective To study the accuracy and specificity of ELISA and colloidal gold strip method in the detection of hepatitis B surface antigen.Methods A total of 460 blood samples were collected from laboratory Department of Maternal and Child Health Hospital from May 2016 to December 2017.ELISA and GICA methods were used to detect and compare the results.Results The positive rate of HBsAg in ELISA was 47.61%,and the positive rate in GICA was 41.96%,the difference was statistically significant(P<0.05).The ELISA method and GICA method have close correlations on the results of surface antigen detection of hepatitis B virus(P<0.05).The sensitivity and specificity of ELISA were higher than those of GICA(P<0.05).Conclusion The ELISA method is more accurate than the GICA method in terms of positive rate,specificity,and sensitivity,but the GICA method is more compact,simple,and intuitive,and does not require large-scale equipment,and is widely used in census and pre-emergency tests.

关 键 词：乙肝表面抗原 胶体金 乙型病毒性肝炎 

分 类 号：R446.6[医药卫生—诊断学]