拟南芥、水稻SUTs基因及其上游序列的系统发生关系  

Phylogenetic Relationship of SUTs Genes and Their Upstream Sequences in Arabidopsis thaliana and Oryza sativa

作　　者：李丹丹 李娟[1,2] 张春龙 徐汝聪 吕东 谭亚玲[1,2] 谭学林[1,2] LI Dandan;LI Juan;ZHANG Chunlong;XU Rucong;LYU Dong;TAN Yaling;TAN Xuelin(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Rice Research Institute of Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学稻作研究所,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2018年第3期381-389,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家重点研发计划(2016YFD0101101)

摘　　要：【目的】对拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)SUTs家族的基因及其上游区的序列进行了核酸组成和系统发生关系的分析,为深入研究该基因作用及其分子调控机制提供参考。【方法】利用邻近法和基因结构分析,解析SUTs基因及其上游序列的系统发生关系。【结果】水稻SUTs基因及其上游区的GC含量都明显高于拟南芥;拟南芥和水稻SUTs基因的序列长度与GC含量呈明显负相关,其基因结构分类与邻近法分类相一致,都可分为3个亚族;拟南芥SUCs上游区的序列长度与其GC含量的相关性不明显,而水稻的SUTs上游区序列长度与其GC含量有较弱的负相关性。此外,拟南芥4个SUC基因(At SUC6~9)上游序列的系统发生关系与基因序列的系统发生完全一致,但其他的At SUCs和Os SUTs基因的上游序列系统发生关系与基因序列的系统发生关系并不一致。【结论】SUTs基因在进化过程中其序列和基因结构相对保守,而其上游序列在进化过程变化较大,揭示了SUTs基因调控序列的复杂性。[Purpose]The phylogenetic relationship analysis between the sequences of SUTs and the upstream sequences of these genes in Arabidopsis thaliana and Oryza sativa provides the reference for further research on the function and molecular regulation of SUTs family.[Method]The phylogenetic relationships of SUTs and their upstream sequences were analyzed by adjacent method and gene structure analysis.[Results]The GC contents of SUTs and their upstream sequences were significantly higher in rice than that in Arabidopsis.The SUTs were divided into three subgroups by genetic structure,which is consistent with the classification of adjacent method.The correlation between the sequence length and GC content of upstream sequence of SUCs in Arabidopsis was not obvious,while the sequence length of the upstream region of SUTs in rice was negatively correlated with its GC content.The phylogenetic relationship of AtSUC6-9 upstream sequences was completely consistent with the phylogeny of genes,but the phylogenetic relationships of upstream sequences of other AtSUCs and OsSUTs were not consistent with that of gene sequences.[Conclusion]SUTs genes were relatively conservative in their sequences and gene structures during evolution,while their upstream sequences change greatly in evolutionary process,revealing the complexity of regulatory sequence of SUTs gene.

关 键 词：SUTs基因 上游序列 基因结构 系统发生 模式植物 

分 类 号：Q756[生物学—分子生物学]