不同产地辣木叶提取物的含量测定及指纹图谱相似性评价  

Simultaneous Determination of 4 Flavonoids and Chlorogenic Acid of Extract from Moringa oleiferam Lam. Leaves and Its UPLC Fingerprint Analysis

作　　者：曾明莹[1] 傅咏梅 张蜀[1] 邓红[1] 王若楠[1] 赖昌威 ZENG Mingying;FU Yongmei;ZHANG Shu;DENG Hong;WANG Ruonan;LAI Changwei(Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems,Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System,Guangdong Pharmaceutical University,Guangzhou 510006,China;Sinopharm Group Guangdong Medi-world Pharmaceutical Co.Ltd.,Foshan 528200,China)

机构地区：[1]广东药科大学,广东省药物新剂型重点实验室,广东省局部精准药物递药制剂工程技术研究中心,广东广州510006 [2]国药集团广东环球制药有限公司,广东佛山528200

出　　处：《云南农业大学学报（自然科学版）》2018年第3期485-493,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：2015广东省普通高校创新团队建设项目(自然科学)(2015KCXTD026)。

摘　　要：【目的】对12批辣木叶提取物指纹图谱进行相似性评价,建立同时测定辣木叶提取物中的绿原酸、维采宁-2、异牡荆素、异槲皮苷和紫云英苷的UPLC分析方法,对12批不同产地辣木叶提取物中的4种黄酮苷和绿原酸进行含量测定。【方法】采用Phenomen Kinetex色谱柱(100 mm×4.6 mm,2.6μm);流动相为乙腈—0.5%甲酸溶液,梯度洗脱,检测波长为334 nm;体积流量0.35 m L/min;柱温30℃。测定12个不同产地辣木叶提取物指纹图谱,采用中药色谱指纹图谱相似度评价系统(2012.130723版)计算相似度,通过对照品和液质联用技术指认色谱峰,并建立外标法测定提取物中的绿原酸及4种黄酮成分(异槲皮苷、紫云英苷、异牡荆素和维采宁-2)含量的UPLC法。【结果】12批辣木叶提取物特征指纹图谱相似度大于0.9,有12个共有峰,指认了其中6个共有峰,即1个酚酸成分[绿原酸(3号峰)]和5个黄酮类成分[维采宁-2(5号峰)、牡荆素(A号峰)、异牡荆素(7号峰),异槲皮苷(8号峰)、紫云英苷(10号峰)];12批辣木叶提取物中异槲皮苷、绿原酸、紫云英苷、维采宁-2和异牡荆素的平均百分含量分别为6.18%、2.28%、2.17%、1.38%、0.32%,5种被测成分总含量最高的产地为广东东莞、广东江门和云南玉溪。【结论】UPLC指纹图谱与含量测定方法准确可行,可为合理评价辣木叶提取物的质量控制提供参考。[Purpose]Our aim was to evaluate the similarity of the ultra performance liquid chromatography(UPLC)fingerprint of extracts from Moringa oleiferam Lam.leaves(MOLL)and to determinate the content of four flavonoid components and chlorogenic acid in extracts of MOLL from 12 different habitats.[Methods]The analysis was carried out on Phenomen Kinetex C18(100 mm×4.6 mm,2.6μm)column.The mobile phase was composed of acetonitrile and 0.5%formic acid water in gradient elution mode.The flow rate was 0.35 mL/min and the detect wavelengths was 334 nm and the column was 30℃.The fingerprints for MOLL extracts from 12 different areas were set up.The similarity assay for MOLL extracts was carried out by Chinese Materia Medica(CMM)Fingerprint Similarity Evaluation System(2012.130723 edition),and the peaks were identified by comparing the reference substance with LC-MS/MS.External standard method was developed to determinate simultaneously contents of four flavonoid components and chlorogenic acid in the extract.[Results]UPLC fingerprints for extract of M.oleiferam leaves were established,which similarity degree was larger than 0.9,six components among twelve common peaks were identified to be chlorogenic acid(peak 3),vicenin-2(peak 5),vitexin(peak A),isovitexin(peak 7),isoquercitrin(peak 8)and astragalin(peak 10)by UPLC-ESI-Q-TOF-MS.The average percentage composition of isoquercitrin,chlorogenic acid,astragalin,vicenin-2 and isovitexin of 12 batches of extract were respectively 6.18%,2.28%,2.17%,1.38%and 0.32%.Top three habitats of the highest gross content of five components are Dongguan and Jiangmen in Guangdong,and Yuxi in Yunnan.[Conclusion]The assay method is reliable and feasible,and provides a reference method for the quantity control method of extracts from M.oleiferam leaves.

关 键 词：辣木叶 指纹图谱 黄酮 含量测定 UPLC 

分 类 号：S794[农业科学—林木遗传育种]