丹参素对腹膜粘连组织成纤维细胞胶原合成与降解的调控  

Regulation of Danshensu on collagen synthesis and degradation of peritoneal adhesion tissue fibroblasts

作　　者：曾煦欣[1] 陈应军[2] 毛建文[3] 王芳[3] 李艳萍[1] 蒋泓[1] 张丽华[1] ZENG Xuxin;CHEN Yingjun;MAO Jianwen;WANG Fang;LI Yanping;JIANG Hong;ZHANG Lihua(School of Stomatology and Medicine,Foshan University,Foshan 528000,China)

机构地区：[1]佛山科学技术学院口腔医学院,广东佛山528000 [2]佛山市第一人民医院,广东佛山528000 [3]广东药科大学,广州510006

出　　处：《实用医学杂志》2018年第13期2128-2131,2136,共5页The Journal of Practical Medicine

基　　金：广东省自然科学基金项目(编号:2015A030313634);广东高校优秀青年创新人才培育项目(编号:LYM10132);广东省高校优秀青年创新人才项目(编号:2014KQNCX182)

摘　　要：目的考察在转化生长因子β1(TGF-β1)刺激下丹参素(DSS)对人体腹膜粘连组织成纤维细胞(ATF)胶原合成与降解的调控作用。方法使用组织块培养法获取ATF,通过CCK-8法确定TGF-β1与DSS的实验剂量,ATF经TGF-β1刺激后加入不同剂量DSS共培养,48 h后收集培养上清液并提取总RNA,分别用ELISA法与Real-time PCR法检测各组的Ⅰ型胶原蛋白(COL-Ⅰ)、基质金属蛋白-1(MMP-1)与基质金属蛋白酶抑制剂-1(TIMP-1)表达。结果 TGF-β1刺激剂量设为10 ng/m L,DSS剂量设为2.5、10和40μmol/L。与模型组比较,DSS各剂量组的COL-ⅠmRNA水平下调,DSS 10、40μmol/L组的TIMP-1 mRNA水平下调,DSS 10、40μmol/L组COL-Ⅰ蛋白水平下降,DSS各剂量组TIMP-1蛋白水平下降,差异均有统计学意义(P<0.01,P<0.05),DSS各剂量组MMP-1蛋白水平升高,但差异无统计学意义(P>0.05)。DSS10、40μmol/L组的COL-Ⅰ蛋白水平以及DSS各剂量组的TIMP-1蛋白水平与空白组比较差异无统计学意义(P>0.05)。结论 DSS通过下调COL-Ⅰ表达抑制ATF胶原合成,下调TIMP-1表达促进ATF胶原降解,并将其COL-I与TIMP-1表达恢复至无TGF-β1刺激的正常水平。Objective To study the effect of Danshensu(DSS)on collagen synthesis and degradation of adhesion tissue fibroblasts(ATF)induced by TGF-β1.Methods ATF from human peritoneal adhesions were harvested by primary culture.CCK-8 was used to confirm the dosage of TGF-β1 and DSS.ATF were stimulated by TGF-β1,then treated with different dosage of DSS.ATF supernatants were collected and total RNA were extracted 48 hours later.ELISA and real-time PCR were employed to determine the expression of COL-Ⅰ,MMP-1 and TIMP-1.Results The stimulating dosage of TGF-β1 was 10 ng/mL.2.5,10 and 40μmol/L of DSS were assigned to co-culture with ATF.COL-Ⅰand TIMP-1 level of DSS groups were down-regulated and there were significant differences between model group and DSS groups(P<0.01,P<0.05).Although DSS groups had higher MMP-1 level than model group,no significant differences were found between groups(P>0.05).Meanwhile,there were no statistically differences in COL-Ⅰand TIMP-1 protein level of model group and DSS groups(P>0.05).Conclusions DSS is a pharmacological agent that can reduce collagen synthesis and promote collagen degradation via down-regulation of COL-Ⅰand TIMP-1 expression in ATF.DSS can also promote over-expression of COL-Ⅰand TIMP-1 induced by TGF-β1 to the normal level.

关 键 词：丹参素 TGF-Β1 腹膜粘连 成纤维细胞 胶原 

分 类 号：R285.5[医药卫生—中药学]