山东省黄瓜细菌性茎软腐病病原菌鉴定与抗病品种筛选  

Pathogen Identification of Cucumber Bacterial Stem Soft Rot in Shandong and Screening of Resistant Varieties

作　　者：熊凯琳 刘文宝 张卫华 方守国[1] 冯传荣 Xiong Kailin;Liu Wenbao;Zhang Weihua;Fang Shouguo;Feng Chuanrong(Yangtze University,Jingzhou 434023,China;Shandong Agriculture and Engineering University,Jinan 250100,China;Insititute of Vegetables and Flowers,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Agricultural Technology Extension Center of Zaozhuang,Zaozhuang 277100,China)

机构地区：[1]长江大学,湖北荆州434023 [2]山东农业工程学院,山东济南250100 [3]山东省农业科学院蔬菜花卉研究所,山东济南250100 [4]枣庄市市中区农技推广中心,山东枣庄277100

出　　处：《山东农业科学》2018年第7期133-137,共5页Shandong Agricultural Sciences

基　　金：山东省科技发展计划项目"蔬菜病毒病快速诊断技术研究及应用"(2014GNC110022);山东省2015农业重大应用创新项目"设施蔬菜根结线虫生态防控关键技术集成与应用"(SDNYZDCX-2015)

摘　　要：本试验以黄瓜细菌性茎软腐病病原菌为研究对象,对黄瓜田间发病情况进行观测,分离病原菌并对其形态、致病性及生理生化特性进行分析测定和分子生物学鉴定,同时利用该菌对36份黄瓜资源进行抗病筛选。结果表明:研究地块黄瓜细菌性茎软腐病发病率为5.62%~43.76%,平均为24.69%。病田中分离得到的黄瓜细菌性茎软腐病病原菌经鉴定为荧光假单胞菌(Pseudomonas fluorescens)。36份黄瓜资源中筛选得到2份高感资源,24份感病资源,10份中抗病资源。In this experiment,with the pathogen of cucumber bacterial stem soft rot as study object,the morbidity situation of cucumber in the field was observed.The morphological,pathogenicity,physiological and biochemical characteristics of the pathogenic bacteria were analyzed and molecular biology identification was conducted.Then the bacteria was used to screen the resistant varieties from 36 cucumber resources.The results showed that the incidence of cucumber bacterial stem soft rot was 5.62%~43.76%,with an average of 24.69%.The pathogen of cucumber bacterial stem soft rot isolated from the disease field was identified as Pseudomonas fluorescens.There were 2 high-susceptive cultivars,24 middle-susceptive cultivars and 10 resistant cultivars in 36 cucumber resources.

关 键 词：黄瓜细菌性茎软腐病 荧光假单胞菌 抗病资源 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]