血府逐瘀汤对血管紧张素Ⅱ诱导大鼠骨髓源内皮祖细胞衰老及p53、SIRT1蛋白表达的影响  

Effects of Xuefu Zhuyu Decoction on Senescence of Rat Bone Marrow-derived EPCs Induced by Angiotensin Ⅱ and Protein Expressions of p53 and SIRT1

作　　者：刘芸[1,2] 谢雪娇 张瑶[3] 谢辉 郭宗耀[1] 张秋雁 梁昊[1] 何莉[1] 杨漾[1] LIU Yun;XIE Xue-jiao;ZHANG Yao;XIE Hui;GUO Zong-yao;ZHANG Qiu-yan;LIANG Hao;HE Li;YANG Yang(Hunan University of Chinese Medicine,Changsha 410208,China;Traditional Chinese Medical Hospital of Chaling County,Chaling 412400,China;The People's Hospital of Ningxiang County,Ningxiang 410600,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]茶陵县中医医院,湖南茶陵412400 [3]宁乡县人民医院,湖南宁乡410600

出　　处：《中国中医药信息杂志》2018年第8期38-43,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81574039)

摘　　要：目的观察血府逐瘀汤对血管紧张素Ⅱ(AngⅡ)诱导大鼠骨髓源内皮祖细胞(EPCs)衰老及对p53、SIRT1蛋白表达的影响,探讨其抗衰老的作用机制。方法体外培养EPCs,加入100 nmol/L AngⅡ诱导细胞衰老,建立EPCs衰老模型,随机分为正常组、模型组、miR-34a抑制剂组、血府逐瘀汤5%药物血清组、血府逐瘀汤10%药物血清组、血府逐瘀汤15%药物血清组。β-半乳糖苷酶染色法检测内皮祖细胞衰老,Western blot检测EPCs中p53蛋白、SIRT1蛋白的表达。结果与正常组比较,模型组EPCs衰老数量明显增多(P<0.01),p53蛋白表达量明显升高(P<0.01),SIRT1蛋白表达量明显降低(P<0.01);与模型组比较,miR-34a抑制剂组和5%、10%、15%药物血清组EPCs衰老数量显著减少(P<0.01),miR-34a抑制剂组和10%、15%药物血清组p53蛋白表达显著降低(P<0.05,P<0.01),miR-34a抑制剂组和10%、15%药物血清组SIRT1蛋白表达明显升高(P<0.05,P<0.01);与miR-34a抑制剂组比较,5%、10%、15%药物血清组EPCs衰老数量显著增多(P<0.05,P<0.01),p53蛋白表达明显升高(P<0.05,P<0.01),SIRT1蛋白表达明显下降(P<0.01);与5%药物血清组比较,10%、15%药物血清组EPCs衰老数量明显减少(P<0.01),p53蛋白表达显著降低(P<0.01),SIRT1蛋白表达显著升高(P<0.01);与10%药物血清组比较,15%药物血清组EPCs衰老数量明显减少(P<0.01),p53蛋白表达差异不明显,SIRT1蛋白表达明显升高(P<0.01)。结论血府逐瘀汤能延缓EPCs的衰老,可明显降低衰老EPCs中p53蛋白的表达,显著增加衰老EPCs中SIRT1蛋白的表达,其中以15%药物血清组效果最佳。Objective To observe the effects of Xuefu Zhuyu Decoction on the senescence of bone marrow-derived endothelial progenitor cells(EPCs)induced by angiotensinⅡ(AngⅡ)and the protein expressions of p53 and SIRT1;To explore its anti-aging mechanism.Methods EPCs were cultured in vitro,and 100 nmol/L AngⅡwas used to induce cell senescence.The model of EPCs senescence was established.The experiment was divided into normal group,model group,miR-34a inhibitor group,5%Xuefu Zhuyu Decoction serum group,10%Xuefu Zhuyu Decoction serum group,15%Xuefu Zhuyu Decoction serum group.β-galactosidase staining was used to detect the senescence of EPCs.The protein expressions of p53 and SIRT1 were detected by Western blot.Results Compared with the normal group,the number of aging EPCs in model group increased significantly(P<0.01);the expression of p53 protein was significantly higher(P<0.01);the expression of SIRT1 protein was significantly decreased(P<0.01).Compared with the model group,the number of aging EPCs in the miR-34a inhibitor group and 5%,10%and 15%Xuefu Zhuyu Decoction serum group was significantly decreased(P<0.01);the expression of p53 protein in miR-34a inhibitor group and 10%and 15%Xuefu Zhuyu Decoction serum groups was significantly decreased(P<0.01);the expression of SIRT1 protein in the miR-34a inhibitor group and 10%and 15%Xuefu Zhuyu Decoction serum groups were up-regulated(P<0.05,P<0.01).Compared with the miR-34a inhibitor group,the number of aging EPCs in 5%,10%and 15%Xuefu Zhuyu Decoction serum groups was significantly increased(P<0.05,P<0.01);the expression of p53 protein were significantly increased(P<0.05,P<0.01);the expression of SIRT1 protein were significantly decreased(P<0.05,P<0.01).Compared with the 5%Xuefu Zhuyu Decoction serum group,the number of aging EPCs in 10%and 15%Xuefu Zhuyu Decoction serum groups was significantly decreased(P<0.01);the expression of p53 protein were significantly decreased(P<0.01);the expression of SIRT1 protein were significantly increased(P<0.01).Compared with the 10%X

关 键 词：血府逐瘀汤 血管紧张素Ⅱ 内皮祖细胞 细胞衰老 P53 SIRT1 蛋白表达 

分 类 号：R285.5[医药卫生—中药学]