海洋动物共附生放线菌分离及拮抗菌筛选  

Separation of Marine Actinomycetes and Screening of Antagonistic Bacteria

作　　者：田云方 傅泽钦 叶莹莹 TIAN Yun-fang;FU Ze-qin;YE Ying-ying(National Engineering Research Center for Marine Aquaculture,Marine Science and Technology Colleage,Zhejiang Ocean University,Zhoushan,Zhejiang 316022)

机构地区：[1]浙江海洋大学海洋科学与技术学院国家海洋设施养殖工程中心,浙江舟山316022

出　　处：《安徽农业科学》2018年第21期1-4,17,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]从舟山常见潮间带海洋动物中分离筛选得到具有产抗菌活性物质的共附生放线菌,采用不同方法测定其抗菌活性和抗菌谱,同时鉴定拮抗菌的分类地位,为研究海洋来源产抗菌物质的菌源提供基础资料。[方法]通过分离纯化技术,从舟山几个潮间带海洋动物中获得放线菌菌株。点种法初筛后获得对金黄色葡萄球菌(Staphylococcus aureus)有一定抑菌作用的菌株;酶标仪法定量测定抑菌活性;滤纸片扩散法复筛获得高效拮抗菌株;采用大肠杆菌(Escherichia coli)、白色念珠球菌(Monilia albican)和枯草芽孢杆菌(Bacillus subtilis)作为指示菌测定其抗菌谱。[结果]对典型菌株GP57和GT16进行生理生化、形态学观察及16S r DNA分子鉴定,其均为链霉菌属(Streptomyces),相似度分别为99%和100%,其中GT16鉴定为灰平链霉菌(Streptomyces griseoplanus)。[结论]海洋共附生微生物中,海洋放线菌可能是一类很好的产抗菌活性物的菌源,值得更进一步的研究。[Objective]Symbiotic or epiphytic actinomycetes with antibacterial active substances were isolated and screened from common intertidal zone marine animals in Zhoushan.Different methods were used for determining the antimicrobial activity and antimicrobial spectrum,the classification status of antagonistic bacteria was also identified for providing basic data for studying the source of marine antibacterial substances.[Method]Through separation and purification technology,strains were obtained from several intertidal zone marine animals in Zhoushan.Strains with certain bacteriostatic effects on Staphylococcus aureus were obtained after initial screening by spot inoculation.Antimicrobial activity was quantitatively determined by enzyme assay.The high-efficiency antagonistic bacteria strains were obtained by filter paper diffusion method.Escherichia coli,Monilia albican and Bacillus subtilis were used as indicators to determine their antimicrobial spectrum.[Result]The typical strains GP57 and GT16 were identified by physiological,morphological and 16S rDNA molecular identification.All of them were Streptomyces with a similarity of 99%and 100%respectively,GT16 was identified as a Streptomyces griseoplanus.[Conclution]Among marine symbiotic or epiphytic microbes,marine actinomycetes may be a very good source of bacteria producing antibacterial actives and deserve further study.

关 键 词：潮间带 海洋动物 共附生微生物 抗菌活性 筛选 

分 类 号：S917[农业科学—水产科学]