天津七里海野生苇蘑菌丝体液体培养基的优化  

Optimization of Liquid Culture Medium of Wild Weimo Mycelium in Qilihai,Tianjin

作　　者：徐营莉 郭成金[1,2] 吕绍生 李兆江 刘西周 XU Ying-li;GUO Cheng-jin;Lü Shao-sheng(College of Life Sciences,Tianjin Normal University,Tianjin 300387;Tianjin Animal and Plant Resistance Key Laboratory,Tianjin 300387;Tianjin Qili Culture Communication Co.,Ltd.,Tianjin 301508)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津市动植物抗性重点实验室,天津300387 [3]天津市七里文化传播有限公司,天津301508 [4]天津师范大学地理与环境科学学院,天津300387

出　　处：《安徽农业科学》2018年第21期12-14,36,共4页Journal of Anhui Agricultural Sciences

摘　　要：以一株分离自天津七里海湿地野生苇蘑为研究对象,以其菌丝体生物量为主要测量指标,对其菌丝体液体培养基进行优化,首先采用不同碳源、氮源进行单因素初选,再结合正交设计,最终得到其菌丝体最佳液体培养基配方为蔗糖20 g/L,蛋白胨4 g/L,Mg SO_4·7H_2O1.5 g/L,KH_2PO_43 g/L,V_(B1)0.004 g/L,p H值自然。29℃培养20 d,菌丝体生物量达到1.980 g/L。A strain of wild weimo isolated from Tianjin Qilihai wetlands was studied in the experiment.The mycelial biomass was choosed as the main index to optimize liquid culture medium of wild weimo mycelium.Firstly,the single factor primary election was conducted with different carbon sources and nitrogen sources,then,in combination with orthogonal design.The optimum liquid nutrient medium was:20 g/L sucrose,4 g/L peptone,1.5 g/L MgSO·7H2O,3 g/L KH2PO4,0.004 g/L vitamin B1,pH natural.Under the condition of dark at 29℃,the biomass of mycelium could reach 1.980 g/L after culturing 20 days.

关 键 词：野生苇蘑 菌丝体 液体培养基 正交设计 生物量 

分 类 号：S646.9[农业科学—蔬菜学]