疏肝补肾法对慢性应激模型小鼠子宫内膜容受性的调节及作用研究  

Study on the regulation of endometrial tolerance and its role in chronic stress model mice

作　　者：赵嘉梅[1] 张爱华[1] 黄紫微 王希浩[1] Zhao Jiamei;Zhang Aihua;Huang Ziwei(Department of Gynaecology,Affiliated Hospital of Henan Institute of Traditional Chinese Medicine,Zhengzhou 450000)

机构地区：[1]河南省中医药研究院附属医院妇科,郑州450000 [2]南京中医药大学,南京210023

出　　处：《陕西中医》2018年第8期991-994,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：河南省基础与前沿技术研究计划项目(142300410058)

摘　　要：目的:研究疏肝补肾中药对于慢性应激模型小鼠子宫内膜容受性的调节及作用机制。方法:将60只SPF级6周龄KM雌性未孕小鼠随机分为4组,每组小鼠数量为15只,即空白对照组(空白组)、应激模型组(模型组)、阳性对照组(逍遥丸组)、中药观察组,除空白组外其余各组均接受21d应激刺激,在刺激期间各组分别灌药,造模成功后各组小鼠按雌雄2∶1合笼,仍接受慢性应激刺激,并给予孕后方药继续灌胃至见栓第5d。观察各组小鼠行为学指标、见栓率、着床数以及子宫内膜VEGF、LIF的表达水平。结果:各组间中央格停留时间、水平穿格数、直立次数、悬尾静止时间均具有统计学差异(P<0.05);经进一步两两对比,各组间各项行为学指标均具有统计学差异(P<0.05)。各组间见栓率具有统计学差异(P<0.05),进一步对比观察组与空白组、模型组与阳性对照组间见栓率无统计学差异(P>0.05);其余各组间均具有统计学差异(P<0.05);观察组胚胎着床数高于其它各组,各组间胚胎着床数两两对比均具有统计学差异(P<0.05)。各组子宫内膜VEGF、LIF表达水平均具有统计学差异(P<0.05)。结论:疏肝补肾法能够有效改善慢性应激小鼠子宫内膜容受性,从而提高妊娠成功率,其作用较单纯应用逍遥丸治疗更为理想。Objective:To study the regulation and action mechanism of traditional Chinese medicine on endometrial tolerance of chronic stress model mice.Methods:Select 60 SPF mice grouped into model group,the observation group and positive control group,on to the next stage experiments;normal feed will not be any processing blank group,model group will not be any drug intervention,positive control group given Xiaoyao pill treatment,observation group with liver kidney in traditional Chinese medicine therapy,treatment after 21 d,snapping the female and male mice according to 2∶1 cage;the blank group and model group maintained normal feeding without any drug intervention,and the positive control group was replaced by the kidney-nourishing pill,and the observation group was changed to the 5d after the pregnancy.The expression levels of the behavioral indicators of the mice in the observation group,the rate of thrombosis,the number of implantation and the Vascular endothelial growth factor(VEGF)and the Leukemia inhibitor(LIF)in the endometrium were observed.Results:There were statistically significant differences in the length of stay between the groups,the number of horizontal penetrations,the number of vertical positions and the duration of suspension(P<0.05);after further comparison,the behavioral indicators of each group were statistically different(P<0.05).There was a statistically significant difference between the two groups(P<0.05),and there was no statistically significant difference between the observation group and the blank group,the model group and the positive control group(P<0.05).There was statistical difference between the other groups(P<0.05);the number of embryo implantation in the observation group was higher than that in other groups,and there were statistically significant differences between the two groups of embryo implantation in each group(P<0.05).The expression levels of VEGF and LIF in each group were statistically significant(P<0.05).Conclusion:This method can effectively improve the endometrial tol

关 键 词：不育 女性/中医药疗法 @疏肝补肾 动物实验 小鼠 

分 类 号：R711.6[医药卫生—妇产科学]