甘草酸二铵对百草枯刺激大鼠Ⅱ型肺泡上皮细胞分泌TGF-β1的影响  

Effects of diammonium glycyrrhizinate on the expression of TGF-β1 in alveolar epithelial cell Ⅱ cultured with paraquat

作　　者：向沥 方辰[1] 周志兵 张剑锋[1] XIANG Li;FANG Chen;ZHOU Zhibing;ZHANG Jianfeng(Department of General Medicine,Second Affiliated Hospital of Guangxi Medical University,Nanning 530007,China)

机构地区：[1]广西医科大学第二附属医院全科医学教研室,南宁530007

出　　处：《实用医学杂志》2018年第14期2320-2323,2328,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81360290);广西自然科学基金项目(编号:桂科计字[2017]198号);广西医疗卫生适宜开发与推广应用项目(编号:S2017009)

摘　　要：目的探讨甘草酸二铵(DG)对百草枯(PQ)刺激大鼠Ⅱ型肺泡上皮细胞(AECⅡ)分泌转化生长因子β1(TGF-β1)的影响。方法采用不同浓度的PQ溶液(200、400、600、800、1 000、1 500、2 000μmol/L)诱导大鼠AECⅡ24 h,测定PQ对AECⅡ生存率的影响,了解IC50的PQ浓度。在1 000μmol/L PQ诱导下,以0、0.2、0.4、0.6、0.8、1.0、2.0 mg/mL浓度DG干预AECⅡ24 h,测定细胞生存率,了解DG干预PQ诱导下AECⅡ最高生存率的DG浓度。再将AECⅡ分为3组,空白组、PQ组(以1 000μmol/L PQ培养24 h)、DG组(先以0.6 mg/m L DG培养2 h,再以0.6 mg/m L DG+1 000μmol/L PQ培养24 h)。倒置显微镜观察AECⅡ的形态及生长情况;酶联免疫吸附法测TGF-β1含量;实时荧光RT-PCR测定TGF-β1 m RNA表达。结果 IC50的PQ浓度为920.87μmol/L,在1 000μmol/L PQ诱导下,以0.6 mg/mL浓度DG干预AECⅡ24 h生存率最高。与空白组比较,PQ组TGF-β1水平及其m RNA表达水平明显升高,差异有统计学意义(P<0.05);与PQ组比较,DG组TGF-β1量及其m RNA的表达明显下降,差异有显著性(P<0.05)。结论本实验成功建立了合适的PQ损伤大鼠II型肺泡上皮细胞的模型及DG干预模型。PQ促进AECⅡ分泌TGF-β1,甘草酸二铵可以降低PQ诱导下AECⅡ分泌TGF-β1水平。Objective To investigate the effects of diammonium glycyrrhizinate(DG)on TGF-β1 in rat alveolar epithelial cellⅡ(AECⅡ)cultured with paraquat(PQ).Methods AECⅡwere cultured with varied con-centrations(200、400、600、800、1 000、1 500、2 000μmol/L)of PQ for 24 h.The cell viability was measured.AECⅡcultured with 1 000μmol/L PQ were treated with varied concentrations(0,0.2,0.4,0.6,0.8,1.0,2.0 mg/mL)of DG for 24 h.The suitable concentration of DG was recorded.The cells were divided into three groups:The NS group,the PQ group(cultured 24 h with 1 000μmol/L PQ),and the DG group(treated with 0.6 mg/mL DG for 2 h,then cultured with 0.6 mg/mL DG+1 000μmol/L PQ for 24 h).Growth status of AECⅡwas observed and the levels of TGF-β1 were measured.The expressions of TGF-β1 mRNA were detected.Results The concentration of PQ at IC50 was 920.87μmol/L.The viability of AECⅡcultured with 1 000μmol/L of PQ was the highest when treated with 0.6 mg/mL of DG;The levels of TGF-β1 and the expression of TGF-β1 mRNA in PQ group were higher than those in NS group.TGF-β1 levels in DG group and the expression of TGF-β1 mRNA were lower than PQ group(P<0.05).Conclusion An appropriate vitro model of AECⅡcultured with PQ and treated with DG are established successfully.We find that DG can decrease TGF-β1 in AECⅡcultured with PQ.

关 键 词：甘草酸二铵 百草枯 Ⅱ型肺泡上皮细胞 转化生长因子Β1 

分 类 号：R595.4[医药卫生—内科学]