外周血CD4^+T细胞Foxp3基因CNS2去甲基化与绝经后骨质疏松症的关系  

Correlation of DNA methylation of CNS2 region of Foxp3 in CD4^+T cells of patients with postmenopausal osteoporosis

作　　者：李子祺[1] 葛鸿庆[1] 王君鳌[1] 王慧敏[1] 胡杏平[1] 陈文治[1] LI Ziqi;GE Hongqing;WANG Junao;WANG Huimin;HU Xingping;CHEN Wenzhi(Department of Orthopedics,Guangdong Provincial Hospital of Chinese Medicine,The Second Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou 510120,China)

机构地区：[1]广东省中医院芳村医院骨科,广州510120

出　　处：《实用医学杂志》2018年第14期2329-2332,共4页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金项目(编号:C2016054)

摘　　要：目的研究绝经后骨质疏松症患者外周血CD4+T细胞Foxp3基因CNS2去甲基化水平。方法纳入绝经后骨质疏松症患者42例为观察组,绝经后无骨质疏松症妇女38例为对照组。检测两组患者血清Ⅰ型前胶原氨基端前肽(P1NP)、β胶原特殊序列(β-crosslaps)以及血钙含量;分离两组患者外周血CD4+T细胞,亚硫酸氢盐测序法检测Foxp3基因CNS2去甲基化水平,实时定量PCR检测Foxp3 m RNA表达量;分析以上指标之间的相关性。结果观察组中Foxp3基因CNS2去甲基化率(P<0.01)、Foxp3基因表达(P<0.01)均显著低于对照组;观察组中P1NP(P<0.01)与β-crosslaps(P<0.01)均显著高于对照组;绝经后骨质疏松症中Foxp3 CNS2去甲基化率与血清β-crosslaps呈负相关,其相关性差异具有统计学意义(P<0.01)。结论 CD4+T细胞中Foxp3 CNS2去甲基化在绝经后骨质疏松症诊断中具有一定价值,其水平与骨代谢异常具有相关性。Objective To investigate the status of DNA methylation of CNS2 region of Foxp3 in CD4+T cells of patients with postmenopausal osteoporosis.Methods Forty-two patients with postmenopausal osteoporosis were enrolled in observational group,and 38 postmenopausal womanwithout osteoporosis as the control group.Serum P1NP,β-crosslaps and Ca2+weredetected.CD4+T cells were isolated from peripheral venous blood.The methylation status of CNS2 region of Foxp3 was detected by bisulfite sequencing PCR.Real-time RT-PCR was used to detect Foxp3 mRNA expression.Results Compared with the control group,levelsofSerum P1NP,β-crosslaps wereobviouslyincreased in patients with postmenopausal osteoporosis.Foxp3 mRNA expression and demethylation of CNS2 region were significantly decreased in CD4+T cell in patients with postmenopausal osteoporosis.A significant negative correlation was found betweenβ-crosslaps and demethylation of CNS2 region.Conclusion DNA hypermethylation of CNS2 region of Foxp3 in CD4+T cells wasapotentialbiomarkerof postmenopausal osteoporosis,which wasassociatedwithboneremodeling.

关 键 词：绝经后骨质疏松症 FOXP3基因 甲基化 

分 类 号：R580[医药卫生—内分泌]