Diascie ProBact全自动微生物分离培养系统临床应用评估  被引量:3

Evaluation of Diascie Pro Bact automated microbial inoculation system

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作  者:郑恬[1] 徐修礼[1] 白露[1] 周柯[1] 陈潇[1] 郝晓柯[1] ZHENG Tian;XU Xiuli;BAI Lu;ZHOU Ke;CHEN Xiao;HAO Xiaoke(Department of Clinical Laboratory,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China)

机构地区:[1]第四军医大学西京医院检验科,陕西西安710032

出  处:《检验医学》2018年第7期644-648,共5页Laboratory Medicine

基  金:陕西省自然科学基础研究计划项目(2014JM4188)

摘  要:目的比较Diascie Pro Bact全自动微生物分离培养系统(简称Diascie Pro Bact)与Copan Wasp全自动微生物前处理系统(简称Copan Wasp)及手工法的微生物分离效果,评估Diascie Pro Bact的临床应用价值。方法收集临床痰、中段尿样本各50例,分别采用Diascie Pro Bact和Copan Wasp及手工法进行划线接种和分离培养。痰样本采用四区划线方式,35℃5%CO_2孵育箱培养;中段尿样本采用连续划线方式,35℃普通孵育箱培养。24 h后观察2种样本的细菌生长情况,并对其分离的菌种数量、细菌生长量、有效单个菌落分离数量进行比较。结果 (1)分离菌种数量。痰样本中Diascie Pro Bact分离0(无细菌生长)、1、2及≥3种菌的样本分别为1、16、8和25例,Copan Wasp分别为1、14、10和25例,手工法分别为2、14、9和25例;中段尿样本中Diascie Pro Bact分离0(无细菌生长)、1、2及≥3种菌的样本分别为20、16、8和6例,Copan Wasp分别为18、17、9及6例,手工法分别为19、18、7和6例。3种方法之间比较,差异均无统计学意义(P>0.05)。(2)细菌生长量。对于痰样本,Diascie Pro Bact分离细菌≤10×10~3、100×10~3、1 000×10~3及≥10 000×10~3 cfu/mL的样本分别为4、5、8和33例,Copan Wasp分别为5、5、9和31例,手工法分别为6、7、10和27例;对于中段尿样本,Diascie Pro Bact分离细菌0(无细菌生长)、10~3~10~4、10~4~10~5和>10~5 cfu/mL的样本分别为20、8、5和17例,Copan Wasp分别为18、9、6和17例,手工法分别为19、7、6和18例。3种方法之间比较,差异均无统计学意义(P>0.05)。(3)有效单个菌落。对于痰样本,Diascie Pro Bact为9.78±5.37,Copan Wasp为10.48±5.59,手工法为8.82±5.31;对于中段尿样本,Diascie Pro Bact为8.78±4.38,Copan Wasp为9.74±4.49,手工法为7.33±5.03。3种方法之间比较,差异均无统计学意义(P>0.05)。结论 Diascie Pro Bact各性能指标与Copan Wasp和手工法无明显差异,可替代手工法应用于临床,以提高菌落分离�Objective To compare the bacterial separation effects of Diascie ProBact automated microbial inoculation system,Copan Wasp automated microbial pretreatment system and manual method,and to evaluate the clinical application significance of Diascie ProBact automated microbial inoculation system.Methods A total of 50 clinical specimens of sputum and 50 clinical specimens of mid-stream urine were inoculated by Diascie ProBact,Copan Wasp and manual method.The sputum specimens were inoculated with a quadrant streaking and incubated at 35℃in 5%CO2 incubator.Mid-stream urine specimens were inoculated with a continuous streaking and incubated at 35℃in common incubator.The bacterial growth of the 2 types of specimens was observed after 24 h.The number of isolate species and single colony isolates and the growth of bacterial colony were analyzed and compared.Results Of 0,1,2 and≥3 isolate species in the sputum specimens,1,16,8 and 25 cases were isolated by Diascie ProBact,1,14,10 and 25 cases by Copan Wasp,and 2,14,9 and 25 cases by manual method,respectively.Of 0,1,2 and≥3 isolate species in the mid-stream urine specimens,20,16,8 and 6 cases were isolated by Diascie ProBact,18,17,9 and 6 cases by Copan Wasp,and 19,18,7 and 6 cases by manual method,respectively.There was no statistical significance among the 3 methods(P>0.05).Of≤10×103,100×103,1 000×103 and≥10 000×103 cfu/mL bacterial quantities in the sputum specimens,4,5,8 and 33 cases were isolated by Diascie ProBact,5,5,9 and 31 cases by Copan Wasp,and 6,7,10 and 27 cases by manual method,respectively.Of 0,103-104,104-105 and>105 cfu/mL bacterial quantities in the mid-stream urine specimens,20,8,5 and 17 cases were isolated by Diascie ProBact,18,9,6 and 17 cases by Copan Wasp,and 19,7,6 and 18 cases by manual method,respectively.There was no statistical significance among the 3 methods(P>0.05).In the sputum specimens,the single colony of Diascie ProBact was 9.78±5.37,that of Copan Wasp was 10.48±5.59,and that of manual method was 8.82±5.31.In the mid-s

关 键 词:自动化 细菌学技术 接种 培养技术 数字成像 

分 类 号:R446.5[医药卫生—诊断学]

 

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