qRT-PCR法分析复发性SLE患者肠道双歧杆菌、柔嫩梭菌数量的变化  

Analysis of the Changes of Intestinal Bifidobacterium and Clostridium Tenella in Patients with Recurrent SLE by qRT-PCR

作　　者：吴霞[1] 肖盼盼 谢晓然 孙雪茜[2] 付冰冰[2] 孙晓鹏[2] WU Xia;XIAO Pan-pan;XIE Xiao-ran;SUN Xue-qian;FU Bing-bing;SUN Xiao-peng(Jiamusi University,Jiamusi 154007,Heilongjiang,China;Department of Rheumatological Immunology,First Affiliated Hospital of Jiamusi University,Jiamusi 154002,Heilongjiang,China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院风湿免疫科,黑龙江佳木斯154002

出　　处：《医学信息》2018年第5期65-69,共5页Journal of Medical Information

基　　金：佳木斯大学研究生科技创新项目(编号:YM2016_030)

摘　　要：目的运用实时荧光定量聚合酶链式反应(q RT-PCR)法分析系统性红斑狼疮患者肠道菌群数量的变化,探讨双歧杆菌、柔嫩梭菌与复发性SLE活动的关系。方法提取粪便细菌组DNA,运用q RT-PCR法分析此二菌绝对数量的变化。结果SLE复发活动期双歧杆菌、柔嫩梭菌数量均较治疗后稳定期减少(P<0.05),对比正常对照组也均减少(P<0.05)。结论双歧杆菌、柔嫩梭菌菌群数量与SLE活动度评分相关,肠道内此二菌数量减少可能与SLE病情的复发有关。Objective To investigate the relationship between bifidobacterium,Clostridium tenella and recurrent SLE activity in patients with systemic lupus erythematosus by real-time quantitative polymerase chain reaction(qRT-PCR).Methods The DNA of fecal bacteria group was extracted,and the absolute quantity of the two bacteria was analyzed by qRT-PCR method.Results Bifidobacterium in the recurrent stage of SLE was found to be bifidobacterium.The number of Clostridium tenella was lower than that of stable period after treatment(<0.05),compared with the normal control group,it was also decreased(<0.05).Conclusion The number of bifidobacterium and Clostridium tenella was correlated with the SLE activity score,and the decrease of these two bacteria in the intestinal tract might be related to the recurrence of SLE.

关 键 词：系统性红斑狼疮 双歧杆菌 柔嫩梭菌 实时荧光定量PCR 

分 类 号：R593.241[医药卫生—内科学]