泛耐药鲍氏不动杆菌氨基糖苷类耐药相关基因研究  

Study on Aminoglycoside Pan Resistance Related Genes of Acinetobacter Baumannii

作　　者：鲍群丽[1] 柯俊[1] 胡芳[1] 张利红 黄艳[1] 程攀[1] 彭紫檀 汪宏良[1] BAO Qun-li;KE Jun;HU Fang;ZHANG Li-Hong;HUANG Yan;CHENG Pan;PEN Zi-Tan;WANG Hong-Liang(Department of Clinical Laboratory,Huangshi Central Hospital,Huangshi 435000,Hubei,China)

机构地区：[1]黄石市中心医院检验科,湖北黄石435000

出　　处：《医学信息》2018年第5期78-80,共3页Journal of Medical Information

基　　金：湖北省黄石市科技局资助项目(编号:黄科技发农[2014]3号55)

摘　　要：目的探讨泛耐药鲍氏不动杆菌氨基糖苷类抗菌药物耐药机制。方法收集2014年湖北省黄石市中心医院住院患者中分离的20株泛耐药鲍氏不动杆菌,用mdf A测序确认菌种,再采用聚合酶链反应法分析16种氨基糖苷类药物获得性耐药相关基因。结果 20株泛耐药鲍氏不动杆菌共检出4种氨基糖苷类获得性耐药基因,氨基糖苷类修饰酶基因aac(3)-Ⅰ阳性率15.00%;ant(3")-Ⅰ阳性率20.00%;aph3'-Ⅰ阳性率100.00%;16Sr RNA甲基化酶未检出;外排泵ade B基因阳性率95.00%。结论泛耐药鲍氏不动杆菌中携带的耐药基因和耐药表型相对应,对氨基糖苷类药物耐药与产aac(3)-Ⅰ、ant(3")-Ⅰ、aph3'-Ⅰ修饰酶基因和获得外排泵ade B 2种耐药机制有关。Objective To investigate the mechanism of aminoglycoside Pan resistance of Acinetobacter baumannii.Methods 20 strains of Pan resistance Acinetobacter baumannii isolated from hospitalized patients in Huangshi central hospital of Hubei province in 2014 were identified by mdfA sequencing.Polymerase chain reaction was used to analyze the acquired resistance related genes of 16 aminoglycosides.Results A total of 4 aminoglycoside-resistant Acinetobacter baumannii strains were detected in 20 Pan-resistant Acinetobacter baumannii strains.The aminoglycoside modifying enzyme gene aac(3)-I was positive in 15.00%;ant(3")-I The positive rate was 20.00%;aph3'-I,the positive rate was 100.00%;16S rRNA methylase was not detected;the positive rate of adeB gene of the efflux pump was 95.00%.Conclusion The drug-resistance genes in pan-resistant Acinetobacter baumannii correspond to drug resistance phenotypes,and they are resistant to aminoglycosides and produce aac(3)-I,ant(3")-I,aph3'-I the modification of the enzyme gene and the acquisition of efflux pump adeB are related to two drug resistance mechanisms.

关 键 词：鲍氏不动杆菌 氨基糖苷类修饰酶基因 外排泵基因 泛耐药 

分 类 号：R378[医药卫生—病原生物学]