基于离子液体型表面活性剂的胶束电动毛细管色谱法分析苏氨酸  

Analysis of Threonine by Micellar Electrokinetic Capillary Chromatography Based on Ionic Liquid Surfactant

作　　者：徐蕾[1] 高立娣[1] 陈林[1] 刘树仁[1] 王宁[1] 李英杰[1] Xu Lei;Gao Lidi ;Chen Lin;Liu Shuren;Wang Ning;Li Yingjie(College of Chemistry and Chemical Engineering,Qiqihar University,Helongjiang Qiqihar 161006)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化工时刊》2018年第6期23-25,50,共4页Chemical Industry Times

基　　金：黑龙江省自然科学基金面上项目(B2016009)资助

摘　　要：利用溴化-1-十二烷基-3-甲基咪唑为胶束电动毛细管色谱的假固定相,建立了对苏氨酸进行测定的方法。详细考察了缓冲溶液pH值及浓度、离子液体型表面活性剂的种类及用量、分离高压、进样条件等因素。最优实验条件如下:以未涂层弹性石英毛细管(55 cm×75μm)为分离通道,25 mmol·L^(-1)的表面活性剂+20 mmol·L^(-1)磷酸盐缓冲溶液(pH=8.0)为运行缓冲溶液,运行高压-25 kV,柱温为23℃,压力进样50 mmbar×40 s。对苏氨酸检测的线性范围为0.01~2 mg·mL^(-1),检出限为5 mg·L^(-1)。结果表明,该方法简单、分析速度快,重现性好。The applicability of an ionic liquid based cationic surfactant 1-dodecyl-3-methylimidazolium bromide(C 12 MImBr)as pseudostationary phase in micellar electrokinetic chromatography(MEKC)has been evaluated for the analysis of threonine.The factors such as pH and concentration of buffer solution,the type and amount of surfactant,the type and amount of ionic liquid,the separation voltage and sample situations were studied in detail.The optimal experimental conditions were achieved as follows,uncoated fused silica capillary(55 cm x 75μm)as the separation channel,25 mmol·L-1 C 12 MImBr+20 mmol·L-1 phosphate buffer solution(pH=8.0)as running buffer,the seperation voltage:25 kV,the column temperature:23℃,the sample hydrodynamic injection:50 mmbar x 40 s.Under the optimum conditions,the calibration curves were linear in the range of 5~200 mg·L-1 and the limit of detection was 1 mg·L-1.The results indicated that this method is simple,fast with a good reproducibility.

关 键 词：离子液体型表面活性剂 胶束电动毛细管色谱 苏氨酸 

分 类 号：O657.7[理学—分析化学] TS202.3[理学—化学]