黄体酮受体对新生大鼠离体延髓脑片呼吸性基本节律放电的调节作用  

Modulated roles of progesterone receptor on the rhythmic respiration discharge activity of medullary slice from neonatal rats in vitro

作　　者：赵华怡 千智斌[1] ZHAO Hua-yi;QIAN Zhi-bin(Functional Lab of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Graduate Student of 2015 in Xinxiang Medical University,Xinxiang 453003,Henan Province,China)

机构地区：[1]新乡医学院机能学实验室,河南新乡453003 [2]新乡医学院,河南新乡453003

出　　处：《新乡医学院学报》2018年第7期568-572,共5页Journal of Xinxiang Medical University

基　　金：河南省科技厅重点攻关项目资助(编号:102102310156)

摘　　要：目的研究延髓面神经后核内侧区(m NRF)神经元细胞膜黄体酮受体对新生大鼠延髓脑片呼吸性基本节律放电(RRDA)的调节作用。方法选择24只2日龄新生大鼠用于制备离体延髓脑片,脑片灌注体积分数95%O2和体积分数5%CO2混合的饱和气体持续通气的人工脑脊液(ACSF),在记录到RRDA后将脑片随机分为4组,每组6个脑片。对照组使用ACSF灌注脑片50 min,以灌流10 min时的数据为对照;黄体酮组分别使用含5、10、20、40μmol·L^(-1)黄体酮的ACSF灌流脑片;米非司酮组分别以含5、10、20、40μmol·L^(-1)米非司酮的ACSF灌流脑片;混合组使用最适浓度黄体酮和最适浓度黄体酮+最适浓度米非司酮联合灌注脑片;比较不同浓度黄体酮、米非司酮及二者联合使用对脑片RRDA的影响。结果对照组、黄体酮组灌注前和5μmol·L^(-1)黄体酮组吸气时程(TI)、吸气幅度(IA)和呼吸频率(RF)比较差异均无统计学意义(P>0.05);与对照组、黄体酮组灌注前和5μmol·L^(-1)黄体酮组相比,10、20、40μmol·L^(-1)黄体酮组TI延长,IA增强,RF增加(P<0.05);与10μmol·L^(-1)黄体酮组比较,20、40μmol·L^(-1)黄体酮组TI延长,IA增强,RF增加(P<0.05);40μmol·L^(-1)黄体酮组与20μmol·L^(-1)黄体酮组TI、IA及RF比较差异均无统计学意义(P>0.05)。对照组、米非司酮组灌注前和5μmol·L^(-1)米非司酮组TI、IA和RF比较差异均无统计学意义(P>0.05);与对照组、米非司酮组灌注前和5μmol·L^(-1)米非司酮组比较,10、20、40μmol·L^(-1)米非司酮组TI缩短,IA减弱,RF减少(P<0.05);与10μmol·L^(-1)米非司酮组比较,20、40μmol·L^(-1)米非司酮组TI缩短,IA减弱,RF减少(P<0.05);40μmol·L^(-1)米非司酮组与20μmol·L^(-1)米非司酮组TI、IA和RF比较差异均无统计学意义(P>0.05)。与对照组和混合组灌注前比较,混合组灌注黄体酮后TI延长,IA增强,RF增加(P<0.05);与灌注黄体酮相比,灌注黄体酮+米非司酮后TI缩短,IA减弱,RF减Objective To investigate the modulated roles of progesterone receptor in the medial region of the nucleus fetrofacialis(mNRF)of the medulla oblongata on the basic rhythmic respiration discharge activity(RRDA)in the medullary respiratory center of neonatal rats.Methods Twenty-four 2 days old neonatal rats were selected to prepare the medullary slice in vitro.The brain slices were perfused artificial cerebrospinal fluid(ACSF)which was continuously ventilated saturation gas with volume fraction of 95%oxygen and volume fraction of 5%carbon dioxide.The brain slices were divided into four groups after the RRDA was recorded,with 6 slices in each group.In control group,the ACSF was perfused into brain slices for 50 min,and the RRDA at 10 min after perfused was taken as control.The brain slices in progesterone group were perfused with ACSF containing 5,10,20,40μmol·L-1 progesterone;the brain slices in mifepristone group were perfused with ACSF containing 5,10,20,40μmol·L-1 mifepristone;the brain slices in mixed group were perfused with optimal concentration of progesterone and optimal concentration of progesterone and mifepristone;the effect of different concentration of progesterone and mifepristone,and the combination of the two drugs on RRDA was observed.Results There was no statistic difference in the inspiratory time(TI),inspiratory strength(IA)and respiratory frequency(RF)among control group,before perfusion of progesterone group and 5μmol·L-1 progesterone group(P>0.05).Compared with the control group,before perfusion of progesterone group and 5μmol·L-1 progesterone group,the TI prolonged,IA enhanced and RF increased in the 10,20,40μmol·L-1 progesterone group(P<0.05).Compared with 10μmol·L-1 progesterone group,the TI prolonged,IA enhanced and RF increased in the 20,40μmol·L-1 progesterone group(P<0.05).There was no statistic difference in the TI,IA and RF between the 20μmol·L-1 progesterone group and 40μmol·L-1 progesterone group(P>0.05).There was no statistic difference in the TI,IA and RF among c

关 键 词：新生大鼠 延髓脑片 黄体酮受体 基本节律性呼吸放电 吸气神经元放电 

分 类 号：R338.2[医药卫生—人体生理学]