琥乙红霉素与茜素红的荷移反应研究  

Charge Transfer Reaction between Erythromycin Ethylsuccinate and Alizarin Red

作　　者：杨振[1] 杨焕 YANG Zhen;YANG Huan(School of Chemisitry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China)

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000

出　　处：《化学与生物工程》2018年第7期61-64,共4页Chemistry & Bioengineering

基　　金：国家级大学生创新创业训练项目(201610722008);陕西省自然科学基金项目(2013JM2016);咸阳师范学院专项科研项目(15XSYK042)

摘　　要：采用紫外分光光度法研究了电子给体琥乙红霉素与π电子受体茜素红的荷移反应,建立了一种测定琥乙红霉素的荷移分光光度法。结果表明,琥乙红霉素与茜素红在乙醇-水(3∶7,体积比)溶液中,室温条件下即可形成1∶1稳定的荷移络合物,该络合物的最大吸收波长为535nm,表观摩尔吸光系数为5.96×103 L·mol-1·cm-1。琥乙红霉素的浓度在8~72μg·mL-1范围内服从比尔定律,R=0.9997。用于片剂中琥乙红霉素的测定时,回收率为97.5%~104.0%,相对标准偏差为1.9%~2.3%(n=5)。该法快速、简便、灵敏、准确,可作为琥乙红霉素的又一测定方法。We studied the charge transfer reaction between erythromycin ethylsuccinate as electron donor and alizarin red asπ-electron acceptor by ultraviolet spectrophotometry.Meanwhile,we established a method of charge transfer spectrophotometry for the determination of erythromycin ethylsuccinate.The results show that the composition ratio of charge transfer complex is 1∶1 in ethanol-water(3∶7)solution at room temperature.The maximum absorption wavelength of the complex is 535 nm,and the apparent molar absorptivity is 5.96×10 3 L·mol^-1·cm^-1.When the concentraion of erythromycin ethylsuccinate is in the range of 8-72μg·mL^-1,the Beer′s Law is obeyed and the correlation coefficient is 0.9997.The recovery is 97.5%-104.0%and the relative standard deviation is 1.9%-2.3%(n=5)in determination of erythromycin ethylsuccinate in tablets.This method is rapid,simple,sensitive,and accurate.

关 键 词：荷移反应 琥乙红霉素 茜素红 分光光度法 

分 类 号：O657.32[理学—分析化学]