循经选穴针刺对失眠大鼠褪黑素的影响  

Effect of Acupuncture with Acupoint Selection Along the Meridians on the Level of Melatonin in Rats with Insomnia

作　　者：魏歆然 魏高文[2] 郑雪娜[2] 吴雪芬[2] 郭鑫[2] 岳增辉[2] WEI Xinran;WEI Gaowen;ZHENG Xuena;WU Xuefen;GUO Xin;YUE Zenghui(The First Affiliated Hospital of Hunan Universty of Chinese Medicine,Changsha,Hunan 410007,China;Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2018年第7期787-790,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2014CB543102)

摘　　要：目的观察固定局部取穴(百会)分别配伍循经(本经、他经、非经)选穴针刺治疗对失眠大鼠生物褪黑素(MT)含量的影响,探索循经选穴对腧穴配伍效应的影响。方法将60只SD雄性大鼠随机分为5组:空白组、模型组、本经穴组(百会+神门组)、他经穴组(百会+三阴交组)、非经非穴组(百会+非经非穴组),每组12只,用腹腔注射对氯苯丙氨酸法(PCPA,500 mg/kg)2 d建立失眠模型,针刺治疗7 d,用ELISA法检测下丘脑腹外侧视前区(VLPO)、视交叉上核(SCN)与血浆中的MT含量。结果与空白组比较,模型组MT含量均降低(P<0.05);与模型组比较,本经穴组、他经穴组MT含量升高(P<0.05);治疗组之间比较,本经穴组高于非经非穴组(P<0.05)。结论固定局部百会穴配伍本经选穴(神门)针刺治疗可以提高失眠大鼠的褪黑素含量,从而提高腧穴配伍效应。Objective To investigate the effect of acupuncture at Baihui acupoint combined with acupoint selection along the meridians(the same meridian,the related meridians,and non-meridians)on the level of melatonin(MT)in rats with insomnia and the effect of acupoint selection along the meridians on acupoint compatibility.Methods A total of 60 male Sprague-Dawley rats were randomly divided into blank group,model group,same meridian group(Baihui and Shenmen),related meridian group(Baihui and Sanyinjiao),and non-meridian/acupoint group(Baihui and non-meridians/acupoints),with 12 rats in each group.A model of insomnia was established by intraperitoneal injection of para-chlorophenylalanine(500 mg/kg)for 2 days,and acupuncture was performed for 7 days.ELISA was used to measure the content of MT in the ventral lateral preoptic area,the suprachiasmatic nucleus,and plasma.Results Compared with the blank group,the model group had a significant reduction in the content of MT(P<0.05);compared with the model group,the same meridian group and the related meridian group had a significant increase in the content of MT(P<0.05);the same meridian group had a significantly higher level of MT than the non-meridian/acupoint group(P<0.05).Conclusion Acupuncture at Baihui acupoint with acupoint selection along the same meridian(Shenmen)can increase the content of MT in rats with insomnia and improve acupoint compatibility.

关 键 词：循经选穴 针刺 失眠 褪黑素 

分 类 号：R256.23[医药卫生—中医内科学]