乌司他丁对兔在体肺缺血再灌注损伤的保护作用  

Investigation of protective effects of ulinastatin on ischemia reperfusion injury of rabbit lung in vivo

作　　者：谷力加[1] 黄邵洪[1] 吴一龙[1] 翁毅敏[1] 冯卫能[1] 程超[1] 钟文昭[1] 

机构地区：[1]中山医科大学附属第三医院胸心外科,广州510630

出　　处：《中华实验外科杂志》2002年第6期585-586,I004,共3页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨乌司他丁对活体肺缺血再灌注损伤的保护作用。方法　将 2 0只新西兰大白兔随机分成缺血再灌注损伤组 (A组 )和乌司他丁组 (B组 ) ,B组阻断前给乌司他丁 (1 0 0 0 0U/kg体重 )。两组阻断 2h和再灌注 1h后采血检测血气、白细胞介素 6(IL 6)和肿瘤坏死因子 α(TNF α)。摘取左肺测定湿干重比 (W/B)和病理学检查。结果　阻断 2h后 ,两组血氧分压 (PO2 )接近 ;恢复灌注 1h后 ,B组PO2 高于A组 (77.61± 5 .0 4 )mmHg(1mmHg =0 .1 33kPa)和 (1 0 0 .85± 6 .73)mmHg ;TNF α值 (A值 ) ,A组各时段均显著高于B组 (2 54 .0 2± 1 4 .31和 50 4 .0 2± 33 .52比 1 4 8.63± 2 1 .0 6)和 1 60 .54± 1 6 .93) ;A组肺湿干重比高于B组 ;肺外观苍白肿胀 ,病理检查见组织结构损伤。Objective To study the protective effects of ulinastatin on ischemia reperfusion lung injury in vivo.Methods Twenty adult Newzeland white rabbits were randomly divided into the groups of ischemia reperfusion lung injury(group A)and the group of ulinastatin (group B).Ulinastatin(10?000?U/kg)was infused before ligating lung hilum in group B.Blood gas analysis was done,and plasma tumor necrosis factor α (TNF α)and inerleukin 6(IL 6)were measured after blocking for 2 h and reperfusion for 1 h.Lung wet to dry weight (W/D)ratio and histological change were observed in the end.Results After blocking for 2 h,PO 2 got close between group A and B,After reperfusion 1 h,PO 2 in group B was higher than group A [(77.61±5.04)?mm?Hg and (100.85±6.73)?mm?Hg)];TNF α (OA)in group A (254.02±14.31,504.02±33.52)was higher than group B (148.63±21.06 and 160.54±16.93) at all time points;Lung W/D ratio in group A was also higher than group B [(8.55±0.61)?g and (5.90±0.55)?g];In morphologic examination group A showed pale,swollen and microstructure destruction.Conclusion Ulinastatin can protect effectively the lesion of lung pathology in ischemia reperfusion in vivo.

关 键 词：乌司他丁 兔 肺缺血 再灌注损伤 保护作用 

分 类 号：R965[医药卫生—药理学]