丁草胺对金鱼主要器官酯酶和过氧化物酶同工酶的影响  

Effect of butachlor on EST and POD isoenzyme in Carassius auratus

作　　者：张晓红[1] 高晋华[1] 范轩坤 陈婉珊 李瑞梅 ZHANG Xiaohong;GAO Jinhua;FAN Xuankun;CHEN Wanshan;LI Ruimei(Department of Biology,Taiyuan Normal University,Taiyuan 030031,Shanxi,China)

机构地区：[1]太原师范学院生物系,山西太原030031

出　　处：《癌变．畸变．突变》2018年第4期286-290,296,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家级大学生创新创业训练计划项目(201610119003);山西省高等学校教学改革项目(J2015096);山西省教育科学"十三五"规划"1331工程"研究专项(ZX-18063);山西省自然科学基金项目(201311031-3)

摘　　要：目的:探讨丁草胺对金鱼主要器官酯酶(EST)和过氧化物酶(POD)同工酶的影响,为评估丁草胺对水生生物的环境风险提供依据。方法:依据丁草胺对金鱼染毒24 h的半数致死浓度设置0.4、0.8、1.2 m L/L丁草胺染毒组,并以0.1 m L/g腹腔注射急性染毒金鱼24 h,采用聚丙烯酰胺凝胶电泳(PAGE)法检测金鱼主要器官的EST和POD同工酶活性。结果:对照组金鱼心脏EST和POD同工酶不表达,在丁草胺1.2 m L/L时产生应激反应,出现新条带,活性较对照组显著升高(P<0.01)。随丁草胺浓度增加,与对照组相比,肝胰脏EST同工酶活性逐渐减弱(P<0.01),POD同工酶活性先增强后减弱(P<0.01)。0.4 m L/L时鳃、肾脏EST同工酶活性分别是对照组的2倍和3倍(P均<0.01);0.8 m L/L时鳃、肾脏EST和POD同工酶活性均消失,在1.2 m L/L时活性重现但较对照组差异明显(P<0.05或P<0.01)。结论:丁草胺浓度与金鱼不同组织EST、POD同工酶活性呈剂量-效应关系。中浓度(0.8 m L/L)及以上丁草胺可能破坏金鱼鳃和肾脏的EST、POD同工酶活性。OBJECTIVE:To explore the environmental risk of butachlor on aquatic organisms.METHODS:0.4,0.8 and 1.2 mL/L concentrations of butachlor were prepared according to 24 h-LC.Carassius auratus were 50 administered by intraperitoneal injection with 0.1 mL/g dose of each concentration for 24 h.PAGE method was used to detect the activity of EST and POD isozyme.RESULTS:EST and POD isozyme in the hearts of C.auratus were not expressed in the control group but was more than 50 times(in 1.2 mL/L)that of the control group.With increased concentrations of butachlor,the activity of EST isozyme in hepatopancreas gradient attenuated while the POD decreased followed by increased.The activities of EST isoenzymes in gill and kidney were 2 times and 3 times higher than those in the control group at 0.4 mL/L,respectively.In addition,the activities of EST and POD isoenzymes in gill and kidney disappeared at 0.8 mL/L and re-expressed at 1.2 mL/L.CONCLUSION:The concentration of butachlor and the activity of EST and POD isozyme in C.auratus show dose-effect relationship.Butachlor with medium concentration(0.8 mL/L)was at risk for EST and POD isozyme in gill and kidney of C.auratus.

关 键 词：丁草胺 金鱼 酯酶 过氧化物酶 

分 类 号：X503.2[环境科学与工程—环境工程]