养殖大菱鲆感染嗜水气单胞菌的分离、毒力分析及ERIC-PCR分型  

Isolation,virulence and ERIC-PCR genotyping of Aeromonas hydrophila in farmed turbot Scophthalmus maximus(L.)

作　　者：徐阳 姚洪[1] 乔帼 李华[1] 叶仕根[1] 黎睿君[1] 李强 XU Yang;YAO Hong;QIAO Guo;LI Hua;YE Shi-gen;LI Rui-jun;LI Qiang(Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China;College of Ocean and Bioengineering,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 [2]盐城工学院海洋与生物工程学院,江苏盐城224051

出　　处：《大连海洋大学学报》2018年第4期430-434,共5页Journal of Dalian Ocean University

基　　金：国家海洋公益性行业科研专项(201405003);辽宁省海洋渔业厅重点攻关项目(201402)

摘　　要：为对辽宁省葫芦岛市养殖患病大菱鲆Scophthalmus maximus(L.)全年感染嗜水气单胞菌Aeromonas hydrophila情况进行调查,通过细菌16S rRNA基因序列分析,以及嗜水气单胞菌特异性引物和气溶素基因的双重PCR扩增对分离株进行鉴定。结果表明:调查中共分离到7株嗜水气单胞菌(AP40301、AP40401、AP40402、AP40403、AP40501、AP40502和AP40503),且均含有气溶素基因(aer A);ERIC-PCR结果进一步显示,7株嗜水气单胞菌存在独立基因型,分别标记为Ⅰ和Ⅱ型,其中Ⅰ型分离株中有3株来自绥中,1株来自兴城,Ⅱ型分离株3株均来自兴城;人工感染试验显示,7株嗜水气单胞菌均具有强致病性,致病率为100%。本研究结果可为养殖大菱鲆的临床诊断以及疾病防控提供理论依据。The isolates from naturally diseased turbot Scophthalmus maximus(L.)in Huludao City,Liaoning Province from 2014 to 2015 were indentified and characterized by 16S rRNA gene sequence analysis and double PCR using Aeromonas hydrophila-specific and aerosol gene primers.The seven isolates were shown to be A.hydrophila,with aerosol genes.The isolates genotyping by ERIC-PCR demonstrated that seven A.hydrophila strains belonged to genotype I and genotype II,in which three strains of type I were isolated from Suizhong farms,one strain of type I from Xingcheng farms and another three strains of type II were from Xingcheng farm.Moreover,the challenge tests by these seven A.hydrophila strains showed that all the strains were highly pathogenic to turbot,with the 7-days cumulative mortality(1.0×10^8 cells/mL)of 100%.The finding provides a theoretical basis for future clinical diagnosis and disease prevention and control of cultured turbot.

关 键 词：大菱鲆 嗜水气单胞菌 毒力分析 ERIC-PCR 

分 类 号：S943[农业科学—水产养殖]