三角帆蚌钩介幼虫寄生胁迫对黄颡鱼营养指标的影响  

Effects of infection stress by glochidia of freshwater mussel Hyriopsis cumingii on main nutritional indices in yellow catfish Pelteobagrus fulvidraco

作　　者：马学艳[1,2] 杜兴伟[1,3] 闻海波[1,2] 金武[1,2] 徐跑[1,2,3] 华丹[2] 顾若波[1,2,3] MA Xue-yan;DU Xing-wei;WEN Hai-bo;JIN Wu;XU Pao;HUA Dan;GU Ruo-bo(Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China)

机构地区：[1]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种与养殖生物学重点开放实验室,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,中美淡水贝类种质资源保护及利用国际联合实验室,江苏无锡214081 [3]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《大连海洋大学学报》2018年第4期472-476,共5页Journal of Dalian Ocean University

基　　金：江苏省自然基金面上项目(BK20161145);国家公益性行业(农业)科研专项(200903028)

摘　　要：为探索三角帆蚌Hyriopsis cumingii钩介幼虫寄生对寄主鱼营养指标的影响,测定了三角帆蚌钩介幼虫寄生胁迫对不同寄生阶段黄颡鱼Pelteobagrus fulvidraco血浆、肝脏和肌肉组织中糖原、游离脂肪酸、总蛋白、游离氨基酸含量的变化情况。结果表明:在相同时间点,寄生组与对照组鱼肝脏和肌肉中糖原含量均无显著性差异(P>0.05);寄生组鱼肝脏中游离脂肪酸含量在寄生后期(12 d)显著低于对照组(P<0.05),在寄生前期(2 d)、中期(5 d)与对照组无显著性差异(P>0.05),寄生组鱼血浆、肌肉游离脂肪酸含量与对照组无显著性差异(P>0.05);寄生组、对照组鱼在寄生中期和后期肝脏总蛋白含量均显著降低(P<0.05),血浆、肌肉总蛋白含量无显著性变化(P>0.05);寄生组鱼血浆游离氨基酸含量在寄生中期和后期显著低于对照组(P<0.05),寄生组鱼肝脏游离氨基酸在寄生后期显著低于对照组(P<0.05),寄生前期、中期与对照组无显著性差异(P>0.05)。研究表明,钩介幼虫寄生会导致肝脏游离脂肪酸降低,血浆及肝脏游离氨基酸含量下降,推测脂肪酸和游离氨基酸可能是钩介幼虫变态发育的关键营养因子。The changes in levels of glycogen,free fatty acid,total protein,and free amino acid were studied in liver,plasma and muscle of yellow catfish Pelteobagrus fulvidraco with body weight of(60.9±0.5)g exposed to parasitic stress of glochidia of freshwater mussel Hyriopsis cumingii at different parasitic stages at water temperature of(24.0±0.5)℃to evaluate the effects of glochidia on nutritional status in the host.The results showed that there was no significant difference in glycogen level in liver in parasitic group and control group at the same time(P>0.05),without significant change in glycogen level in muscles(P>0.05).The yellow catfish had significantly lower free fatty acid level in liver in the parasitic group than the fish in the control group did in later parasitism(12 d)(P<0.05),without significant difference in earlier stage(2 d)and middle stage(5 d).There was no significant difference in free fatty acid level in serum and muscle in the parasitic group than that in the control group(P>0.05).The significant decrease in total protein level was observed in liver in both parasitic group and control group in middle and later parasitism(P<0.05),without significant changes in total protein level in plasma and muscle(P>0.05).The yellow catfish in parasitic group had significantly lower free amino acid in plasma in middle and later parasitism than the fish in the control group(P<0.05),significantly lower free amino acid level in liver in later parasitism than the fish in the control group did(P<0.05),without significant difference in earlier and middle parasitism(P>0.05).The findings indicate that the glochidia leads to decline in levels of free fatty acid in liver,free amino acid content in plasma and liver,indicating that fatty acids and free amino acids might be the key nutrition factors for the development of glochidium.

关 键 词：三角帆蚌 钩介幼虫 寄生 营养指标 

分 类 号：S966.22[农业科学—水产养殖]