免疫组化染色中不同抗原修复方法的应用探讨  

Application of different antigen retrieval methods in immunohistochemical staining

作　　者：魏素姣 Wei Sujiao(Department of Pathology,Luoyang Central Hospital Affiliated to Zhengzhou University,Luoyang 471003,China)

机构地区：[1]郑州大学附属洛阳中心医院病理科,471003

出　　处：《国际医药卫生导报》2018年第13期2000-2003,共4页International Medicine and Health Guidance News

摘　　要：目的 主要探讨高压锅与微波炉抗原修复方式在免疫组化染色中的应用效果。方法 以本院2016年8月至2017年6月收集的12例食管癌组织标本作研究对象,选用定位于细胞膜、细胞核、细胞浆的CD4、CD8、Ki67、IL17四种不同抗原做染色标记,并应用高压锅与微波炉两种抗原修复方式,对两种抗原修复法应用免疫组化染色结果进行分析。结果 在高压锅抗原修复方式下,CD4抗体阳性率高达100%,而在微波炉抗原修复方式下,CD4抗体阳性率仅达45.5%,两组相较而言,高压锅抗原修复法CD4抗体阳性率较高(P<0.05);CD8、Ki67、IL17抗体阳性率对比差异无统计学意义(P>0.05)。结论 在组织不脱片情况下,选用高压锅抗原修复方式,操作简单,背景着色浅,定位清晰,结果稳定;在修复方式阳性率比较无明显差异情况下,建议选用微波抗原修复方式。Objective To discuss the application effect of different antigen retrieval methods with autoclave and microwave oven in immunohistochemical staining.Methods 12 cases of esophageal cancer tissue specimens in our hospital from August 2016 to June 2017 were collected for the study,four different antigens(CD4,CD8,Ki67,IL17)located in the cell membrane,nucleus,cytoplasm were selected to do staining,two antigen retrieval methods with autoclave and microwave oven were performed.The immunohistochemical staining results of the two antigen retrieval methods were analyzed.Results The antibody positive rate of CD4 of the antigen retrieval method with autoclave was as high as 100%,which was higher than that of the antigen retrieval method with microwave oven(45.5%)(P<0.05);there were no statistically significant differences in the antibody positive rates of CD8,Ki67,and IL17(P>0.05).Conclusion In the case of non-shedding tissue,the antigen retrieval method with autoclave has the advantages such as simple operation,light background coloring,clear positioning,stable result;in the case of no significant difference in the positive rate of retrieval cases,the microwave antigen retrieval method is proposed.

关 键 词：免疫组化染色 抗原修复 高压锅 微波炉 

分 类 号：R392[医药卫生—免疫学]