三七对脑出血合并VTE大鼠血小板活化因子的影响  

Effect of pseudo-ginseng on platelet activating factor in rats with cerebral hemorrhage complicated with venous thromboembolism

作　　者：邝晶 张志荣[2] 祝晨[2] 蔡丹莉[2] 王灵聪[2] KUANG Jing;ZHANG Zhirong;ZHU Chen(The First Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学第一临床医学院,浙江杭州310053 [2]浙江中医药大学附属第一医院ICU

出　　处：《全科医学临床与教育》2018年第4期372-376,481,共6页Clinical Education of General Practice

基　　金：浙江省自然科学基金(LY17H29006);浙江省卫生高层次创新人才培养工程项目资助(2014-108)

摘　　要：目的探索三七对脑出血合并VTE大鼠的血小板活化因子的影响。方法将50只大鼠按随机数字表法分为空白对照组、假手术组、脑出血合并VTE模型组、阳性对照组、三七组(浓度为2.5 g/kg),每组10只,分别采取相应处理及造模;在脑出血建模成功后24 h对大鼠行头颅MRI,VTE造模后48 h结扎血管组织进行苏木精-伊红(HE)染色,并采用流式检测分析血小板活化因子CD61及CD62P表达。结果大鼠头颅MRI示三七组大鼠出血量、血肿程度和梗死面积明显比模型组少或减轻;大鼠结扎血管组织HE染色镜下示模型组血管栓塞严重,而三七组血管栓塞明显好转。各组大鼠脑组织中CD61的表达量无明显差异(F=2.76,P>0.05),与空白对照组比较,模型组、阳性对照组和三七组大鼠脑组织中CD62P的表达量明显上调(t分别=-2.79、-2.06、-3.38,P均<0.05);与模型组比较,三七组大鼠脑组织中CD62P的表达量明显上调(t=-0.59,P<0.05)。各组大鼠结扎血管段组织中CD61的表达量无明显差异(F=1.76,P>0.05),与空白对照组比较,模型组、阳性对照组和三七组大鼠结扎血管段组织中CD62P的表达量明显上调(t分别=-7.69、-5.73、-4.37,P均<0.05);与模型组比较,三七组大鼠结扎血管段组织中CD62P的表达量明显下调(t=3.32,P<0.05)。结论三七能一定程度上双向调节血小板活化因子CD61和CD62P的表达,对脑出血合并VTE大鼠有一定改善作用。Objective To explore the effect of pseudo-ginseng on platelet activating factor in rats with cerebral hemorrhage complicated with VTE.Methods Totally 50 rats were randomly divided into blank control group(blank),cerebral hemorrhage combined with VTE sham operation group(sham),cerebral hemorrhage combined with VTE model group(model),positive control group(fraxiparine),and the pseudo-ginseng group(with the concentration of 2.5 g/kg)with 10 cases in each.24 hours after brain hemorrhage model established,all rats from five groups accepted head MRI.And 48 hours after VTE model established,the HE staining was taken to the ligature vascular tissue.The flow cytometry were used to detected the expression quantity of platelet activating factor CD61 and CD62P.Results The head MRI showed that the intracerebral hemorrhage volume,the distribution of haematomas and the sizes of the infarct of the pseudo-ginseng group were less or smaller than the model group.And the HE staining of the ligature blood vessels tissues showed that the model group had serious vascular embolism,while the pseudo-ginseng group was obviously improved.There was no significant difference in the expression of CD61 in the brain tissue of rats among each group(F=2.76,P>0.05).Compared with the blank control group,the expressions of CD62P in the brain tissues of the rats in model group,the positive control group and the pseudo-ginseng group were obviously increased(t=-2.79,-2.06,-3.38,P<0.05).The expression of CD62P of the pseudoginseng group was significantly higher than that of the model group(t=-0.59,P<0.05).There was no significant difference in the expression of CD61 in the ligature tissue of rats among each group(F=1.76,P>0.05).Compared with the blank control group,the expressions of CD62P in the ligated vascular segments of the model group,the positive control group and the pseudo-ginseng group were significantly increased(t=-7.69,-5.73,-4.37,P<0.05).Compared with the model group,the expression of CD62P of pseudo-ginseng group was significantly decrease

关 键 词：三七 脑出血 静脉血栓栓塞症 CD61 CD62P 

分 类 号：R285.5[医药卫生—中药学]