小麦-野生亲缘种属添加系白粉病新抗源筛选及特异分子标记鉴定  

Identification of Powdery Mildew Resistance Resources from Wheat-Wild Relative Disomic Addition Lines and Development of Molecular Markers of Alien Chromosome-Specialty

作　　者：夏晴[1] 麦艳娜 董振杰 刘文轩[1] XIA Qing;MAI Yanna;DONG Zhenjie;LIU Wenxuan(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业科学》2018年第6期64-69,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31571658);河南省科技重大专项(161100110400)

摘　　要：为从小麦亲缘种属发掘新的白粉病抗性基因,利用河南省小麦白粉病混合流行菌株对100份小麦-野生亲缘种属添加系进行苗期抗性鉴定,从中筛选出6份抗白粉病材料,并将抗病基因分别定位在沙融山羊草4S^(sh)#8,高大山羊草3S^l#3、4S^l#3、6S^l#3,尾状山羊草E#1,两芒山羊草2M^(bi)#1染色体上。除3S^l#3外,其余5条外源染色体所携带的抗白粉病基因均为新基因。此外,利用小麦表达序列标签(EST)和全长cDNA序列开发了106个STS和FlcDNA分子标记,从中筛选出12个携带抗白粉病基因外源染色体特异的分子标记,其中STS标记2个,FlcDNA标记10个。这些特异分子标记可用于进一步筛选小麦-野生亲缘种属外源染色体易位系,及向小麦转移抗白粉病新基因。In order to explore and utilize alien resistant genes of powdery mildew derived from wild relatives of wheat,100 wheat-wild relative disomic addition lines were screened for the resistance against powdery mildew at seedling stage using mixed isolates of Blumeria graminis f.sp.tritici collected in Henan province.Of them six lines against powdery mildew were identified,and the resistant genes were located on chromosomes 4S sh#8 of Aegilop sharonensis,3S l#3,4S l#3 and 6S l#3 of Ae.longissima,E#1 of Ae.caudata,and 2M bi#1 of Ae.biuncialis,respectively.All the resistant genes except the one located on 3S l#3 of Ae.longissima were novel genes against powdery mildew.Furthermore,a total of 106 molecular makers were designed based on expressed sequence tags(EST)and full-length cDNA(FlcDNA)of wheat,and 12 markers,of which two were STS(sequence tagged site)type and the remaining 10 were FlcDNA type,were confirmed to be specific for alien chromosomes carrying novel resistant genes against powdery mildew.These specific markers can be used for further selection of wheat-alien chromosome translocations and transfer of alien resistant genes identified in our research into common wheat.

关 键 词：小麦 小麦-野生亲缘种属添加系 白粉病 新抗源 分子标记 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]