黄瓜氮素胁迫相关基因CsAHP1的克隆及功能分析  

Cloning and Function Analysis of Cucumber CsAHP1 Gene Involved in Nitrogen Tolerance

作　　者：杜亚琳 陈海燕 徐丽琳 王琛 范莲雪 DU Yalin;CHEN Haiyan;XU Lilin;WANG Chen;FAN Lianxue(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture/Horticulture and Landscape Architecture College of Northeast Agricultural University,Harbin 150030,China;Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]农业部东北地区园艺作物生物学与种质创制重点实验室/东北农业大学园艺园林学院,黑龙江哈尔滨150030 [2]河南省农业科学院农业经济与信息研究所,河南郑州450002

出　　处：《河南农业科学》2018年第6期92-97,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金(31701934);东北农业大学/农业部东北地区园艺作物生物学与种质创制重点实验室开放课题(neauhc201601)

摘　　要：为研究黄瓜耐低氮素胁迫的生理与分子机制,克隆了1个黄瓜氮素胁迫相关基因CsAHP1,其cDNA全长为462 bp。序列分析表明,CsAHP1基因在不同物种中高度保守。进化树分析结果表明,CsAHP1蛋白与甜瓜的AHP蛋白亲缘关系较近。qRT-PCR结果表明,CsAHP1基因在低浓度氮素条件下表达量较高。功能分析结果表明,低浓度氮素胁迫条件下拟南芥Col-0植株根长明显变短,其质量也显著降低,而35S∶∶CsAHP1转基因拟南芥株系的根长缩短程度和质量下降率都显著小于Col-0植株,表明CsAHP1过表达拟南芥转基因植株表现出比Col-0植株更强的耐氮素胁迫能力。综上所述,黄瓜CsAHP1基因可以提高35S∶∶CsAHP1拟南芥过表达转基因株系的氮素胁迫能力。To investigate the physiological and molecular mechanism of low nitrogen(N)tolerance in cucumber,a low N tolerance related gene,named CsAHP 1 was cloned in the present study.The cDNA of CsAHP 1 was 462 bp in length.Sequence analysis showed that CsAHP 1 gene was highly conserved among different species.Phylogenetic analysis indicated that CsAHP1 had a close relationship with Cucumis melo AHP among different species.qRT-PCR analysis results showed that CsAHP 1 was up-regulated under low N condition.Functional analysis results showed that root lengths and fresh weights of Col-0 plants were significantly reduced under low N condition,while reduction ratio of root lengths and fresh weights in transgenic line harboring 35S∶∶CsAHP 1 was less than Col-0,which revealed that transgenic Arabidopsis plants showed higher capacity for low N tolerance than Col-0.These results revealed that the cucumber CsAHP 1 could improve low N tolerance in transgenic Arabidopsis plants.

关 键 词：拟南芥 CsAHP 1 黄瓜 低氮素胁迫 

分 类 号：S642.2[农业科学—蔬菜学]