MicroRNA-21对肺高压血管平滑肌细胞增殖的影响  

Role of Micro RNA-21 on proliferation of vascular smooth muscle cells in pulmonary hypertension

作　　者：吴翔[1] 卓书伟 郑才玲 高戈[2] Xiang Wu;Shu-wei Zhuo;Cai-ling Zheng;Ge Gao(Clinical Laboratory,Hainan Provincial Hospital of Chinese Medicine,Haikou,Hainan 570203,China;Department of Laboratory Medicine,Central South University,Changsha,Hunan 410013,China)

机构地区：[1]海南省中医院检验科,海南海口570203 [2]中南大学医学检验系,湖南长沙410013

出　　处：《中国现代医学杂志》2018年第21期28-31,共4页China Journal of Modern Medicine

摘　　要：目的研究micro RNA-21(mi RNA-21)在Wnt/β-catenin信号通路中抑制肺高压(PH)血管平滑肌细胞增殖的可能机制。方法复制外源性mi RNA-21干扰的野百合碱大鼠肺高压原代肺动脉平滑肌细胞(PASMCs)模型,分别用Western blot和实时荧光定量PCR检测外源性mi RNA-21对Wnt通路中β-catenin、Cyclin D1和拮抗剂DDK1表达的影响。结果外源性抑制mi RNA-21表达时,PASMCs中β-catenin、Cyclin D1表达水平增高,拮抗剂DDK1表达降低,拮抗作用减弱;而外源性上调mi RNA-21表达时,PASMCs中β-catenin、Cyclin D1表达水平降低,拮抗剂DDK1表达增高,拮抗作用增强。结论mi RNA-21的早期干预可通过调控Wnt通路的拮抗剂DKK1作用与Wnt/β-catenin信号通路参与PH肺血管重构。Objective To study the role of MicroRNA-21 on proliferation of pulmonary vascular smooth muscle cells in pulmonary hypertension and potential mechanisms.Methods Rat model of monocrotaline-induced pulmonary hypertension(PH)was established.Primary pulmonary artery smooth muscle cells were isolated and treated with various insults.Western blot and Real-time fluorescent quantitative PCR were performed for determination of expression ofβ-catenin,Cyclin D1 and antagonist of DDK1.Results The level ofβ-catenin and Cyclin D1 was increased while DDK1 was decreased significantly when exogenous MicroRNA-21 was inhibited.However,upregulation of exogenous MicroRNA-21 induced decreased levels ofβ-catenin and Cyclin D1 increased levels of antagonist of DDK1.Conclusions Early intervention of MicroRNA-21 participates in pulmonary remodeling in PH model through regulating Wnt/β-catenin signaling pathway.

关 键 词：MIRNA-21 肺高压 WNT通路 增殖 血管重构 

分 类 号：R544.1[医药卫生—心血管疾病]