2,4-二氯苯氧乙酸作用的鼠乳对幼鼠蓝斑核中多巴胺β-羟化酶表达的影响  

Effect of 2, 4-Dichlorophenoxyacetic acid on expression of Dopamine beta hydroxylase in locus coeruleus of neonate rats

作　　者：李京 毛庆花 秦燕霞 Jing Li;Qing-hua Mao;Yan-xia Qin(College of Basic Medicine,Jining Medical University,Jining,Shandong 272067,China;Department of Neonatal Intensive Care Unit,Jining First People’s Hospital,Jining,Shandong 272000,China)

机构地区：[1]山东省济宁医学院基础医学院,山东济宁272067 [2]山东省济宁市第一人民医院新生儿科,山东济宁272000

出　　处：《中国现代医学杂志》2018年第21期32-35,共4页China Journal of Modern Medicine

基　　金：山东省医药卫生科技发展计划项目(No:2016WS0161);2015年济宁医学院科研计划项目(No:JY2015KJ009)

摘　　要：目的观察经腹腔注射除草剂2,4-二氯苯氧乙酸(简称2,4-D)的鼠乳,对幼鼠蓝斑核中多巴胺β-羟化酶(DBH)表达的变化,探讨除草剂对神经系统发育的影响。方法生后7 d且经母乳喂养的幼鼠30只,分成实验组1(10只)、实验组2(10只)及对照组(10只)。实验组1、2分别按照每天70及100 mg/kg剂量注射2,4-D至母鼠腹腔(二甲亚砜0.1 ml做溶剂);对照组只进行正常鼠乳喂养。待幼鼠哺乳至第22天,分别测量其体重、脑重;取幼鼠的蓝斑核进行DBH免疫染色。结果 (1)至生后22 d,实验组2的幼鼠体重(35.60±2.84)g与对照组比较(47.21±2.90)g降低26.8%(P=0.000);而脑重分别为(1.16±0.05)和(1.33±0.04)g,实验组2降低13.1%(P=0.000);(2)实验组1、2幼鼠蓝斑核DBH相对光密度值为(0.21±0.03)和(0.19±0.02),低于对照组(0.88±0.38)(P=0.000)。结论 2,4-D能够抑制幼鼠体重增加;降低幼鼠蓝斑核DBH的表达,进而减少脑内去甲肾上腺素的合成,抑制脑及神经系统发育。Objective To explore the effects of 2,4-Dichlorophenoxyacetic acid(2,4-D)on expression of Dopamine beta hydroxylase(DBH)in the locus coeruleus(LC)rats.Methods A total of 307-days-old neonatal rats were randomly divided into 3 groups:group 1 in which maternal rats received 70 mg/kg/day of 2,4-D for 14 consecutive days(n=10),group 2 in which maternal rats received 100 mg/kg/day of 2,4-D for14 consecutive days(n=10),and control group in which maternal rats did not receive any insults(n=10).All neonatal rats were breast feeding.Offsprings were sacrificed 22 days after birth and midbrains were harvested for immunohistochemistry staining.Results Rats in group 2 showed a significant reduction of body weight and brain weight when compared with control group(35.60±2.84 g vs 47.21±2.90 g,P=0.000,1.16±0.05 g vs 1.33±0.04 g,P=0.000,respectively).Expression of DBH decreased significantly in group 1 and group 2 when compared with control group(0.21±0.03 vs 0.88±0.38,0.19±0.02 vs 0.88±0.38,P=0.000,respectively).Conclusion 2,4-D inhibits the activity of DBH,leading to the decrease of Noradrenaline in the LC of the pups and damage to the nervous system.

关 键 词：2 4-二氯苯氧乙酸 多巴胺-Β-羟化酶 蓝斑核 幼鼠 

分 类 号：R322.7[医药卫生—人体解剖和组织胚胎学]