逍遥丸对雷公藤多苷致大鼠排卵障碍模型卵巢氧化损伤和颗粒细胞凋亡的影响  

Effect of Xiaoyao Pill on Ovarian Oxidative Damage and Apoptosis in Glycosides of Tripterygium Wilfordii-induced Ovulation Obstacles Rats

作　　者：周楠[1] 杨宏敏[2] 马惠荣[3] 回丽敏 刘振兴[1] 杜惠兰[3] ZHOU Nan;YANG Hongmin;MA Huirong;HUI Linmin;LIU Zhenxing;DUI Huilan(Xingtai People's Hospital,Hebei Xingtai 054031,China;Hebei Medical University,Hebei Shijiazhuang 050031,China;Hebei University of Chinese Medicine,Hebei Shijiazhuang 050200,China)

机构地区：[1]邢台市人民医院,河北邢台054031 [2]河北医科大学,河北石家庄050031 [3]河北中医学院,河北石家庄050200

出　　处：《中国药物警戒》2018年第7期389-392,共4页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金(30772818):补肾法和疏肝法对生殖功能影响特征的比较研究

摘　　要：目的观察逍遥丸对雷公藤多苷致排卵障碍模型大鼠卵巢氧化损伤和颗粒细胞凋亡的影响。方法建立雷公藤多苷致排卵障碍大鼠模型,136只Wistar大鼠随机分为空白对照组、模型组、逍遥丸高剂量组和低剂量组,每组34只。比色法测定血清和卵巢组织中Tbil、Dbil和T-AOC的含量,硫代巴比妥法测定MDA水平;末端脱氧核糖核苷酸转移酶介导的缺口末端标记(terminal deoxynucleotidyl transferase-mediated nick-end labeling,TUNEL)法检测卵巢颗粒细胞的凋亡水平。结果与空白对照组比较,模型组大鼠血清及卵巢组织中Tbil、Dbil和T-AOC水平明显降低(均P<0.01),MDA明显升高(均P<0.01),卵巢颗粒细胞的凋亡率明显增加(P<0.01)。与模型组比较,逍遥丸(54、27 g·kg-1)明显提高大鼠血清和卵巢组织中Tbil、Dbil和T-AOC水平(均P<0.05),降低卵巢组织中MDA水平(均P<0.05)及颗粒细胞的凋亡(P<0.01),其中逍遥丸高剂量组药效最佳。结论逍遥丸通过改善雷公藤多苷致排卵障碍模型大鼠的卵巢氧化损伤和颗粒细胞的凋亡,从而治疗排卵障碍性相关疾病。Objective To observe the effects of Xiaoyao pill(XYP)on ovarian oxidative damage and apoptosis in Glycosides of Tripterygium Wilfordii(GTW)-induced ovulation obstacles rats.Methods The rat model of ovulation caused by GTW was successfully established.One hundred and thirty-six rats were randomly divided into control group,model group,XYP high-dose group and XYP low-dose group,with 34 rats in each group.Serum and ovarian tissues levels of Tbil,Dbil and T-AOC were detected by colorimetry,and the levels of MDA were detected by penthiobarbital.Apoptosis of ovarian granulosa cells was observed by TUNEL.Results Compared with control group,serum and ovarian tissues MDA level increased(P<0.01),while Tbil,Dbil and T-AOC levels decreased in the model group(all P<0.01),the apoptosis of ovarian granulosa cells was significantly increased in model group(P<0.01).After treatment with XYP,the levels of serum and ovarian tissues Tbil and Dbil were increased(all P<0.05),while the levels of ovarian tissues MDA in those groups were lower than model group(all P<0.05).Moreover,the apoptosis of ovarian granulosa cells was significantly lower than those in model group(all P<0.01).XYP high-dose group shows the best effect.Conclusion XYP could improve follicular development and ovulation obstacles by regulating ovarian oxidative damage and apoptosis.

关 键 词：逍遥丸 雷公藤多苷致大鼠排卵障碍模型 氧化损伤 细胞凋亡 卵巢 

分 类 号：R965[医药卫生—药理学]