香菊片对变应性鼻炎大鼠行为和鼻黏膜水通道蛋白3表达影响  

The Effect of Xiangju Tablet on Behavior of Rats with Allergic Rhinitis and Expression of Aquaporin 3 in Nasal Mucosa

作　　者：黄中官 白洪亮 叶纪拓 苏倩 HUANG Zhong-guan;BAI Hong-liang;YE Ji-tuo;SU Qian(Department of Otorhinolaryngology Head and Neck Surgery,the People's Hospital of Pingyang,Pingyang,Zhejiang 325400,China;Shaanxi Xiangju Pharmaceutical Group Co.,Ltd.,Chinese Medicine Technology R&D Center,Shangluo,Shaanxi 726000,China)

机构地区：[1]平阳县人民医院耳鼻咽喉头颈外科,浙江平阳325400 [2]陕西香菊药业集团有限公司中药技术研发中心,陕西商洛726000

出　　处：《临床误诊误治》2018年第8期101-104,共4页Clinical Misdiagnosis & Mistherapy

基　　金：2013年浙江省卫生厅一般项目(2013KYB300)

摘　　要：目的探讨香菊片对变应性鼻炎大鼠行为和鼻黏膜水通道蛋白3(aquaporin 3,AQP3)表达的影响。方法选取36只健康SD大鼠随机将其分为正常组、模型组、阳性对照组、香菊片低剂量组(鼻饲香菊片50 mg/kg)、香菊片中剂量组(鼻饲香菊片100 mg/kg)和香菊片高剂量组(鼻饲香菊片200 mg/kg)6组各6只。模型组、阳性对照组、香菊片低剂量组、香菊片中剂量组和香菊片高剂量组均建立变应性鼻炎模型。阳性对照组采用85 mg/kg的氯雷他定灌胃给药,不同剂量香菊片组给予相应剂量香菊片鼻饲,正常组和模型组以等体积的蒸馏水来代替药物灌胃。连续灌胃10 d后,观察比较各组在15 min内的抓鼻次数和打喷嚏次数,并比较各组鼻黏膜AQP3表达情况及灰度值。结果连续灌胃10 d后,各组大鼠打喷嚏次数、抓鼻次数和AQP3表达灰度值总体比较差异均有统计学意义(P<0.05)。连续灌胃10 d后,与正常组比较,模型组打喷嚏次数和抓鼻次数显著增加,差异具有统计学意义(P<0.05)。连续灌胃10 d后,与模型组比较,阳性对照组与香菊片低剂量组、香菊片中剂量组、香菊片高剂量组抓鼻次数和打喷嚏次数较低,且随着香菊片剂量的增加,抓鼻次数和打喷嚏次数不断减少,差异有统计学意义(P<0.05)。连续灌胃10 d后,香菊片中剂量组和香菊片低剂量组的抓鼻次数和打喷嚏次数显著高于阳性对照组,差异有统计学意义(P<0.05)。与正常组比较,模型组AQP3表达灰度值明显降低,差异有统计学意义(P<0.05)。与模型组比较,阳性对照组和不同剂量香菊片组AQP3表达灰度值均升高,且随着香菊片浓度增加,AQP3表达灰度值呈现不断增加趋势,差异均具有统计学意义(P<0.05)。香菊片中剂量组和香菊片低剂量组AQP3表达灰度值显著低于阳性对照组,差异有统计学意义(P<0.05)。结论香菊片可能通过调节AQP3在变应性鼻炎大鼠鼻黏膜的表达,缓解变应�Objective To study the effect of Xiangju tablet on behavior of rats with allergic rhinitis and expression of Aquaporin 3(AQP3)in nasal mucosa.Methods A total of 36 healthy rats were randomly divided into normal group,model group,positive control group,low-dose Xiangju tablet group(low-dose group,50 mg/kg)medium-dose Xiangju tablet group(medium-dose group,100 mg/kg)and high-dose Xiangju tablet group(high-dose group,200 mg/kg),with 6 rats in each group.The allergic rhinitis model was established in the model group,positive control group,low-dose group,medium-dose group and high-dose group,respectively.In the positive control group,85 mg/kg of loratadine was intragastrically administered.Low-dose,medium-dose and high-dose groups were given the corresponding dose of Xiangju tablets by nasal feeding.The normal group and the model group were treated intragastrically with an equal volume of distilled water instead of drugs.After continuous gastric gavage for 10d,the times of sniffling and sneezing within 15 min were recorded,and the expression of AQP-3 and its grayscale value was observed and compared in the nasal mucosa of each group.Results After gastric gavage for 10d,there were significant differences in the times of sneezing and sniffling and the overall value of AQP3 expression in rats(P<0.05).After 10 d of gastric gavage,the times of sniffling and sneezing of model group were significantly higher than those in the normal group(P<0.05).Compared with the model group,the times of sniffling and sneezing were lower in low-dose,medium-dose and high-dose group and the positive control group,and with the increase in the dose of Xiangjue tablets,the times of sniffling and sneezing had been decreasing,and the difference was statistically significant(P<0.05).After gastric gavage for 10d,the times of sniffling and sneezing in low-and medium-dose group in Xiangju tablets was significantly higher than those in the positive control group(P<0.05).Compared with the normal group,the value of gray scale ratios of AQP3 expression in

关 键 词：鼻炎 大鼠 香菊片 水通道蛋白3 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]