青蒿琥酯对人骨髓瘤细胞抗血管生成的作用  

Anti-angiogenesis effect of artesunate on human multiple myeloma cells

作　　者：师亮[1] 陈浩[1,2] 崔慧林 张皓洁[1] 郝跃亭 Shi Liang;Chen Hao;Cui Huilin;Zhang Haojie;Hao Yueting(Department of Histology and Embryology,Shanxi Medical University,Taiyuan 030001;Affiliated Dayi Hospital of Shanxi Medical University,Shanxi Academy of Medical Sciences,Taiyuan 030026,China)

机构地区：[1]山西医科大学组织学胚胎学教研室,太原030001 [2]山西医学科学院,山西医科大学附属大医院,太原030026

出　　处：《解剖学杂志》2018年第2期152-155,共4页Chinese Journal of Anatomy

基　　金：山西省自然科学基金(2015021189);山西医科大学博士启动基金(03201410);山西医科大学基础医学科技培植基金(201423);山西医科大学大学生创新创业基金(20172098)

摘　　要：目的:研究青蒿琥酯对人RPMI-8226细胞诱导血管新生的抑制作用。方法:采用MTT法检测青蒿琥酯对RPMI-8226细胞增殖抑制作用;通过鸡胚绒毛尿囊膜体内血管生长实验,观察青蒿琥酯对RPMI-8226细胞诱导体内血管生成的影响;免疫蛋白印迹检测鸡胚绒毛尿囊膜内血管内皮生长因子(VEGF)和血管紧张素1(Ang-1)含量的表达变化。结果:青蒿琥酯以时间、剂量依赖方式抑制RPMI-8226细胞增殖;24h和48h后,其IC50值分别为(36.33±2.65)μmol/L和(14.31±3.28)μmol/L。在鸡胚绒毛尿囊膜体内血管生长实验中,经3、6、12μmol/L青蒿琥酯处理后,与单纯RPMI-8226细胞组比较,新生血管数目分别减少21.9%、38.2%和76.9%,差异有统计学意义;同时鸡胚绒毛尿囊膜内VEGF含量分别显著下降22.2%、34.2%和52.6%;Ang-1蛋白表达量分别显著下降15.6%、24.2%和39.6%。结论:青蒿琥酯具有抑制RPMI-8226细胞诱导血管新生的作用,其作用机制与下调RPMI-8226细胞的VEGF和Ang-1表达有关。Objective:To study the effect of artesunate on human myeloma RPMI-8226 cell-induced angiogenesis.Methods:The proliferation inhibition effect of artesunate on RPMI-8226 cells was evaluated by MTT method.The chorioallantoic membrane(CAM)vascular assay in chick embryo was used to examine the effect of artesunate on the angiogenesis in vivo induced by RPMI-8226 cells.After CAM vascular assay,the vascular endothelial growth factor(VEGF)and angiotensin-1(Ang-1)protein content of CAMs was detected by Western blotting.Results:Artesunate inhibited the human RPMI-8226 cell proliferation in a time and dose dependent manner.IC 50 of 24 and 48 hours after artesunate treatment was(36.33±2.65)μmol/L and(14.31±3.28)μmol/L,respectively.Compared with the RPMI-8226 cell group,the number of neovascularization in 3,6 and 12μmol/L artesunate treatment groups was reduced by 21.9%,38.2%and 76.9%,respectively.At the same time,the expression of VEGF protein in the CAMs was decreased by 22.2%,34.2%and 52.6%,respectively,and the expression of Ang-1 was decreased by 15.6%,24.2%and 39.6%,respectively.Conclusion:Artesunate can inhibit the human multiple myeloma RPMI-8226 cell-induced angiogenesis,which is related to the VEGF and Ang-1 down-regulation of RPMI-8226 cells after artesunate treatment.

关 键 词：多发性骨髓瘤 青蒿琥酯 血管新生 血管内皮生长因子 血管紧张素1 

分 类 号：R285.5[医药卫生—中药学]