大建中汤治疗大鼠脾阳虚胃炎的机制  

Mechanism of Dajianzhong decoction in treating gastritis with spleen-Yang deficiency

作　　者：武静[1] 杨莎莎[2] 陈继婷[1] 王俊霞[1] 杨毅[1] 龚小雪[1] 王慧[1] Wu Jing;Yang Shasha;Chen Jiting;Wang Junxia;Yang Yi;Gong Xiaoxue;Wang Hui

机构地区：[1]贵阳中医学院,贵阳550002 [2]成都中医药大学,成都610045

出　　处：《解剖学杂志》2018年第2期156-159,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(81360590);贵州省教育厅青年科技人才成长项目(黔教合KY字[2017]170);贵州省中医药管理局项目(QZYY2013-109);贵阳中医学院大学生创新创业训练计划项目(贵中医大创合字(2016)21号)

摘　　要：目的:探讨大建中汤治疗脾阳虚胃炎的作用机制。方法:SD大鼠,随机分为正常对照组、模型组、枸橼酸铋钾对照组,大建中汤高、中、低剂量组。采用RT-PCR检测单核细胞白介素-1受体相关激酶-4(IRAK-4)mRNA表达水平;ELISA法检测血清中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α);同时观察胃黏膜的病理。结果:与正常对照组相比,模型组大鼠IRAK-4mRNA和血清中IL-6、TNF-α的水平升高,胃黏膜显示腺萎缩、浸润;与模型组比较,大建中汤高剂量组、中剂量组IRAK-4mRNA和血清中IL-6、TNF-α均降低,差异有统计学意义;大建中汤低剂量组血清中IL-6、TNF-α差异亦显著;大建中汤低剂量组IRAK-4mRNA无明显差异。结论:大建中汤高剂量组、中剂量组均对脾阳虚胃炎有显著治疗作用,大建中汤降低IRAK-4、IL-6及TNF-α可能是治疗胃炎的机制之一。Objective:To explore the mechanism and to clarify the scientific connotation of Dajianzhong decoction in treating gastritis with spleen-Yang deficiency.Methods:Sixty SD rats were randomly divided into blank control group,gastritis with spleen-Yang deficiency model group,bismuth potassium citrate control group,Dajianzhong high-dose group,Dajianzhong middle-dose group and Dajianzhong low-dose group.Expression of IL-1 receptor-related kinase-4(IRAK-4)mRNA in monocytes was analyzed by RT-PCR.Levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the serum were detected by ELISA.Results:Compared with the blank control group,the level of IRAK-4 mRNA in the blood mononuclear cells and the levels of IL-6 and TNF-αin the serum of rats in the model group were higher.In the model group,the gastric mucosa showed chronic inflammation,atrophy of mucous gland,focal erosion of lymphocytes on the natural layer,formation of lymph follicles and thickening of the mucosa.Compared with the model group,the level of IRAK-4 mRNA in the blood mononuclear cells and the levels of IL-6 and TNF-αin the serum of rats in the bismuth potassium citrate control group,Dajianzhong high-dose group and Dajianzhong middle-dose group were decreased significantly.The difference of IL-6 and TNF-αin serum of Dajianzhong decoction low dose group was significant.There was no significant difference of IRAK-4 mRNA in Dajianzhong decoction low dose group.Conclusion:Dajianzhong decoction can reduce the levels of IRAK-4,IL-6 and TNF-αin the high-dose group and middle-dose group,which may be one of the mechanisms of gastritis treatment.

关 键 词：大建中汤 脾阳虚胃炎 白介素-1受体相关激酶-4 白细胞介素-6 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]