NADPH氧化酶p22phox亚基C242T基因多态性与中国朝鲜族2型糖尿病患者下肢动脉硬化的相关性  

Association between NADPH oxidase subunit p22phox gene C242T polymorphism and lower limb arteriosclerosise in type 2 diabetic patients

作　　者：金光明 朴莲善[2] JIN Guangming;PIAO Lianshan(Department of Diagnostic Radiology,Yanbian University Hospital,Yanji 133000,China)

机构地区：[1]延边大学附属医院医学影像二科,吉林延吉133000 [2]延边大学附属医院内分泌科,吉林延吉133000

出　　处：《实用医学杂志》2018年第15期2510-2513,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:30460053)

摘　　要：目的探讨NADPH氧化酶p22phox亚基C242T基因多态性与中国朝鲜族2型糖尿病(T2DM)患者下肢动脉硬化(LLAS)的相关性。方法选取诊断为T2DM的中国朝鲜族患者298例,彩色多普勒超声观察患者LLAS情况,将存在LLAS的患者分为无斑块和有斑块组,记录全部T2DM患者血液生化指标。同时,应用聚合酶链反应-限制性片段长度多态性技术对所有T2DM患者的NADPH氧化酶p22phox亚基242位点进行基因型分析。结果比较无斑块和有斑块两组,NADPH氧化酶p22phox亚基242位点上CC基因与CT及TT基因基因频率存在显著性差异(P<0.05);Logistic回归分析显示NADPH氧化酶p22phox亚基C242T基因多态性与病程及吸烟同为下肢动脉硬化斑块(LLASP)形成的危险因素;纯合子CC基因型LLASP的风险高于杂合子CT及纯合子TT基因型患者。结论 NADPH氧化酶p22phox亚基C242T基因多态性与中国朝鲜族T2DM患者并发LLAS相关,其中CC纯合子基因是中国朝鲜族T2DM患者并发LLASP的遗传学风险因素。Objective To investigate the correlation of the NADPH oxidase subunit p22phox gene C242T polymorphism with lower limb arteriosclerosise(LLAS)in Chinese Korean type 2 diabetic patients.Methods A total of 298 consecutive patients of Korean nationality with diagnosed T2DM in our hospital from October 2015 to October 2017 were enrolled in this research.They were all observed with color doppler ultrasound for lower limb arteriosclerosise,then the patients with arteriosclerosise were divided into two groups:plague group and non-plague group.The blood biochemical indexes of patients were recorded.NADPH oxidase subunit p22phox gene C242T polymorphism was analyzed by restriction fragment length polymorphism(RFPL).Results The distributions of CC genotype vs.CT and TT genotype were significantly different in NADPH oxidase subunit p22phox gene 242 between Korean T2DM arteriosclerosise patients without plagues and with plaques.Logistic analysis showed that NADPH oxidase subunit p22phox gene C242T polymorphism,disease couse and smoking were risk factors for arteriosclerosise plaques.CC homozygous genotype was more riskier than the CT heterzygous and TT homozygous genotype for which the patients developed into arteriosclerosise plaques.Conclusions NADPH oxidase subunit p22phox gene C242T polymorphism is related with Korean T2DM arteriosclerosise patients complicated with LLAS.The CC genotype is susceptible for the Korean T2DM patients with arteriosclerosise plaques.

关 键 词：NADPH氧化酶p22phox亚基 基因多态性 2型糖尿病 下肢动脉硬化 彩色多普勒超声 

分 类 号：R587.1[医药卫生—内分泌]