海洋真菌Rhodotorula mucilageinosa GIM 2.157介导邻-、间-、对-溴苯乙酮的不对称还原反应  

Asymmetric bioreduction of o-, m-, and p-bromo acetophenone by whole cells of marine-derived fungi

作　　者：刘慧 杨振男 刘岚 陈碧双[1,2] LIU Hui;YANG Zhen-nan;LIU Lan;CHEN Bi-Shuang(School of Marine Sciences,Sun Yat-Sen University,Guangzhou 510275,China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center,Sun Yat-Sen University Guangzhou 510275,China)

机构地区：[1]中山大学海洋科学学院,广东广州510275 [2]中山大学南海资源开发与保护协同创新中心,广东广州510275

出　　处：《海洋科学》2018年第3期106-112,共7页Marine Sciences

基　　金：高校基本科研业务费中山大学青年教师培育项目(17lgpy58);国家自然科学基金(41706148);广东省自然科学基金(2017A030310232);海洋经济创新发展区域示范专项(2018ZX09735010)~~

摘　　要：研究了海洋真菌介导邻-、间-、对-溴苯乙酮的不对称还原反应。具体考察了Rhodotorulamucilageinosa GIM 2.157对邻-、间-、对-溴苯乙酮的催化活性和对映选择性,并综合考察了反应温度、pH、反应时间和底物浓度等因素对还原产物的产率及立体选择性的影响。确定最佳反应条件:反应温度为25℃,反应体系的pH为7,反应时间为24 h,底物浓度为10 mmol/L。在最佳条件下,产率以及立体选择性均>99%。In this study,the catalytic activity and enantioselectivity of Rhodotorula mucilageinosa GIM 2.157 toward the bioreduction of o-,m-,and p-bromoacetophenone to chiral alcohols were investigated.The effects of reaction temperature,pH,reaction time,and substrate concentration on the yield and enantioselectivity of the product,(S)-1-(4-bromophenyl)ethanol,were evaluated.The determined optimum reaction conditions are as follows:25℃reaction temperature,reaction system pH of 7,24 h reaction time,and 10 mmol/L substrate concentration.Under the optimum conditions,the desired chiral alcohols were achieved with yield and ee up to>99%.

关 键 词：海洋真菌 邻-、间-、对-溴苯乙酮 不对称还原 生物催化 

分 类 号：P745[天文地球—海洋生物学]