2016～2017年辽宁地区猪圆环病毒2型分子流行病学调查及全基因组进化分析  

Molecular epidemiological investigation and complete genomic sequence analysis of porcine circovirus type 2 in Liaoning from 2016 to 2017

作　　者：梁乔[1,2] 张健 申贯男[1,2] 王宏燕 关淼[1,2] 杨洺扬 李蓉[1,2] Liang Qiao;Zhang Jian;Shen Guaunan;Wang Hongyan;Guan miao;Yang Mingyang;Li Rong(Liaoning Center for Animal Disease Control and Prevention,Liaoning Shenyang 110164;Animal Medicine Research Institute of Liaoning Province,Liaoning Shenyang 110164)

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110164 [2]辽宁省动物医学研究院,辽宁沈阳110164

出　　处：《现代畜牧兽医》2018年第7期45-49,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：为了解近几年辽宁猪圆环病毒2型(PCV2)的流行趋势和流行特点,对辽宁地区14个市共3 430份屠宰场样品采用荧光PCR方法进行检测,其中,2016年为1 580份,2017年为1 850份。并通过PCR方法对7株PCV2阳性病料进行全基因组扩增,并用DNA Star软件进行序列比对和分析。病原学检测结果表明:两年间PCV2病原学阳性率分别为83.54%、85.62%,呈上升趋势;测序结果表明:7株PCV2毒株基因核酸序列同源性为93.9%~100%,其中,有4株属于PCV2d基因型,2株属于PCV2a基因型,1株属于PCV2b基因型。In order to understand the epidemic trend and characteristics of porcine circovirus type 2(PCV2)in Liaoning Province in recent years,A total of 3 430 slaughterhouse samples from 14 cities in Liaoning Province were detected by RT-PCR method.The year of 2017 was 1 580,and the The year of 2018 was 1 850.The whole genome of 7 strains of PCV2 positive venereal diseases were amplified by PCR,and the sequence alignment and analysis were carried out by DNAStar software.The results of etiological detection showed that:The positive rates of PCV2 pathogen were 83.54%and 85.62%in two years,respectively.The sequencing results showed that the nucleic acid sequence homology of the PCV2 strain of 7 strains was 93.9%~100%.Among them,4 strains belonged to genotype PCV2d and 2 strains belonged to genotype PCV2a,another belonged to genotype PCV2b.

关 键 词：猪圆环病毒2型 病原学检测 序列分析 

分 类 号：S858.28[农业科学—临床兽医学]