HPLC-UV法测定鸦胆子油乳注射液中甘油三酯油酸酯的含量  

Qantitation of triacylglycerols in Brucea Fructus by HPLC-UV

作　　者：邓延秋 唐红珍[1] 张振伟 DENG Yan-qiu;TANG Hong-zhen;ZHANG Zhen-wei(Guangxi University of traditional Chinese Medicine,Guangxi Nanning 530200,China)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《临床医药文献电子杂志》2018年第51期7-9,共3页Electronic Journal of Clinical Medical Literature

基　　金：广西高校人文社科重点研究基地:中国-东盟传统医药发展研究中心(编号:J17009);广西中医药大学中药学优势学科建设专项课题(编号050170035)

摘　　要：目的建立一种使用高效液相-紫外法测定鸦胆子油乳注射液中指标性成分甘油三油酸酯含量的方法。方法色谱柱为Waters symmetry C18(150 mm×4.6 mm,5μm);以乙腈-异丙醇(55:45)为流动相,流速1 ml/min,检测波长为205 nm;柱温25℃。结果甘油三酯油酸酯在0.529~10.58μg范围内线性良好,r=0.9999,平均加样回收率(n=9)为99.7%,RSD为0.9%。结论本法采用高效液相色谱法,结合常规的紫外检测器,建立了一种简便可靠的鸦胆子油乳注射液中甘油三油酸酯的含量测定方法,该方法专属性强,重复性好,为鸦胆子油乳注射液的质量控制提供一种选择和参考。Objective To establish a qantitation of triacylglycerols in Brucea Fructus by high performance liquid chromatography with UV detector.Methods Waters symmetry C18 column(4.6 mm×250 mm,5μm),Acetonitrile and iso-propanol(55:45)as mobile phase,the flow rate is 1 ml/min,the detection wavelength is 205nm,the column temperature is 25℃.Results The linear range of triacylglycerols is 0.529~10.58μg(r=0.9999),the average recovery rate is 99.7%,(n=9,RSD=0.9%).Conclusion A simple and reliable HPLC-UVmethod is established for the qantitation of triacylglycerols in Brucea Fructus with UV detector.

关 键 词：高效液相色谱-紫外法 鸦胆子油乳注射液 甘油三油酸酯 含量测定 

分 类 号：R927.2[医药卫生—药学]