‘涌金’丛生芽的增殖优化研究  

Study Experiment on Optimization of Propagation for Clump Buds of Cinnamomum camphora ‘Yongjin'

作　　者：张椿芳[1] 付涛[1] 何月秋[1] 王建军 王豪 郑国强 ZHANG Chun-fang;FU Tao;HE Yue-qiu;WANG Jian-jun;WANG Hao;ZHENG Guo-qiang(Ningbo City College of Vocational Technology,Ningbo 315100,Zhejiang,China;Seedling Breeding Center of Ningbo Specialty Forest Seedlings,Ningbo Forestry Bureau,Ningbo 315012,Zhejiang,China;Jiangsu Bi Yun Tian Agriculture and Forestry Science and Technology Co.,Ltd.of Jiangsu,Danyang 212300,China)

机构地区：[1]宁波城市职业技术学院,浙江宁波315100 [2]宁波林业局林特种苗繁育中心,浙江宁波315012 [3]江苏碧云天农林科技有限公司,江苏丹阳212300

出　　处：《浙江林业科技》2018年第3期1-8,共8页Journal of Zhejiang Forestry Science and Technology

基　　金：国家星火计划项目(2015GA690062);宁波农业重大(重点)项目(2012C10013)

摘　　要：为了提高‘涌金’Cinnamomum camphora‘Yongjin’丛生芽的增殖效率及生长状况,以其尚未萌发的腋芽新梢作为实验材料,通过植物组织培养技术对不同培养基、不同激素、不同蔗糖浓度、不同p H值、不同继代方式以及不同继代周期进行了系统研究。结果表明,‘涌金’丛生芽增殖优化的最优方案为MS+Na H2PO4(50 mg·L^(-1))+6-BA(2 mg·L^(-1))+IBA(0.02 mg·L^(-1)),蔗糖浓度在20~30 g·L^(-1)之间,p H值5.8~6.2,继代方式为(1)-(3)-(1)[(1):MS+Na H_2PO_4(50 mg·L^(-1))+6-BA(3 mg·L^(-1))+IBA(0.02 mg·L^(-1));(3):MS+Na H2PO4(50 mg·L^(-1))+6-BA(1 mg·L^(-1))+IBA(0.02 mg·L^(-1))],继代周期在25~30 d之间。本研究有效改善了‘涌金’继代培养过程中丛生芽长势衰弱、黄叶、褐化等问题,提高了增殖效率。New shoots were collected from 14-year Cinnamomum camphora‘Yongjin’in Ningbo,Zhejiang province in March of 2015 for experiment on propagation of clump buds.In order to improve the proliferation efficiency and growth condition of clump buds in C.caphora‘Yongjin’,new shoots of axillary buds that have not yet germinated was as experimental materials of the‘Yongjin’in this paper,systematic study was carried out about different medium,hormones,sucrose concentrations,pH values,subculture patterns and succession subculture cycles by means of plant tissue culture techniques was tested on growth of clump buds.The result showed that the optimal scheme propagation of clump buds was as follows:MS+50 mg·L^-1 of NaH2PO4+2 mg·L^-1 of 6-BA+0.02 mg·L^-1of IBA,about multiplication optimization of clump buds in‘Yongjin’,and sucrose concentration was between 20-30 g·L^-1,pH was 5.8-6.2,subculture pattern was①-③-①(①:MS+50 mg·L^-1 of NaH2PO4+3 mg·L^-1 of 6-BA+0.02mg·L^-1 of 6-IBA,③:MS+50 mg·L^-1 of NaH2PO4+1 mg·L^-1 of 6-BA+0.02 mg·L^-1 of IBA,subculture cycle was of 25-30 days.The results of this study can effectively improve growing weakness,yellow,brown of the buds in‘Yongjin’subculture,and increase the proliferation efficiency.

关 键 词：'涌金’ 丛生芽 组织培养 增殖 

分 类 号：S792.23[农业科学—林木遗传育种]