中药对高脂血症性重症急性胰腺炎大鼠肠黏膜通透性及甘油三酯影响  

Effect of Chinese Herbs “Wenli Gongxia” Method on Intestinal Permeability and Triglyceride in Rats with Hyperlipidemic Severe Acute Pancreatitis

作　　者：伍彦辉 田玉芝[2] 董韶华 杨丽 WU Yan-hui;TIAN Yu-zhi;DONG Shao-hua(Fifth Hospital of Shijiazhuang,Shijiazhuang(050021),China)

机构地区：[1]石家庄市第五医院,石家庄050021 [2]河北医科大学第三医院,石家庄050051 [3]邢台医专第二附属医院,邢台054000

出　　处：《中国中西医结合外科杂志》2018年第4期461-465,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：河北省中医药管理局项目(2013017)

摘　　要：目的:探讨"温里攻下法"中药对高脂血症性重症急性胰腺炎(HLSAP)大鼠肠黏膜通透性及甘油三酯的影响。方法:100只雄性SD大鼠按照随机数字表法,分为对照组、SAP组、HLSAP组、SAP治疗组和HLSAP治疗组共5组,每组20只,逆行胆胰管注射牛黄胆酸钠建立大鼠SAP模型,继续股静脉注射Triton Wr1339建立HLSAP模型,两治疗组于造模前2 h给予胰炎合剂灌胃,各组在设定时段中检测尿中乳果糖和甘露醇排泄率的比值(L/M)、血清甘油三脂(TG)和血清淀粉酶水平。结果:各时段SAP组及HLSAP组大鼠L/M逐渐升高,24 h、36 h达最高峰,该两组相比,HLSAP组L/M高于SAP组,但无显著性差异(P>0.05),与对照组相比差异具有统计学意义(P<0.05);HLSAP治疗组和SAP治疗组与SAP组及HLSAP组相比,在建模后时段12 h时段L/M无明显差异24 h、36 h时段SAP治疗组L/M明显低于SAP组(P<0.05),HLSAP治疗组明显低于HLSAP组(P<0.05)。术后各时段HLSAP组及HLSAP治疗组TG水平明显高于SAP组、SAP治疗组和对照组(P<0.05);24 h、36 h时段HLSAP治疗组TG下降水平最明显(P<0.05)。各时间段SAP组、HLSAP组、SAP治疗组及HLSAP治疗组血清淀粉酶水平明显高于对照组(P<0.05);24、36 h时段SAP治疗组及HLSAP治疗组血清淀粉酶水平分别较SAP组及HLSAP组有明显下降(P<0.05)。结论:高脂血症会增加SAP大鼠肠屏蔽功能障碍,胰炎合剂能改善大鼠肠黏膜通透性和肠屏障功能,可降低其TG水平。Objective To investigate the effect of“Wenli Gongxia”Chinese medicine method on intestinal permeability and triglyceride in rats with hyperlipidemic severe acute pancreatitis.Methods A total of 100 male SD rats were randomly divided into control group,severe acute pancreatitis group(SAP group),high fat blood hyperlipidemic severe acute pancreatitis group(HLSAP group)and SAP and HLSAP treatment group,(n=20,in each group).The urinary lactulose and mannitol exhaust discharge rate ratio(L/M)and serum triglyceride(TG)level were detected.Results The L/M of SAP group and HLSAP group increased gradually,and reached the highest peak after 24 h and 36 h.The L/M in HLSAP group was higher than that of SAP group,and there was no significant difference(P>0.05).The difference was statistically significant compared with the control group(P<0.05).In HLSAP treatment group and SAP treatment group,compared with SAP and HLSAP groups,after the modeling time and 12 h,no obvious difference was found between L/M.After 24 h and 36 h,the L/M levels in two treatment groups were obviously lower than those of SAP group and HLSAP group,difference had statistical significance(P<0.05).The SAP treatment group was significantly lower than SAP group(P<0.05).HLSAP treatment group was significantly lower than HLSAP group(P<0.05).In HLSAP group and HLSAP treatment group,the levels of TG were significantly higher than those in SAP group,SAP treatment group and contrast group(P<0.05).After 24 and 36 h,the levels of TG in each group were decreased significantly(P<0.05)and HLSAP treatment group decreased the level most obviously(P<0.05).The serum amylase levels in SAP group and HLSAP group were significantly higher than those in the control group at each time point(P<0.05).At 24 and 36 h after treatment,SAP treatment group and HLSAP treatment group were significantly lower than those of SAP group and HLSAP group(P<0.05).Conclusion Hyperlipidemia can aggravate the intestinal barrier dysfunction in severe acute pancreatitis,and the effect of Chinese her

关 键 词：温里攻下法 高脂血症性重症急性胰腺炎 肠黏膜通透性 甘油三酯 

分 类 号：Q95-33[生物学—动物学] R657.51[医药卫生—外科学]