抗病毒病K326新品系Y48抗TMV的细胞学机制研究  

Analysis of Cytological Mechanism of TMV Resistance in the Novel Anti-Viral Disease Variety Y48 Developed from K326

作　　者：文柳璎[1] 刘旦[1] 龚敏 马冰[1] 申莉莉[1] 罗成刚[1] 任民[1] 程立锐[1] 蒋彩虹[1] 杨爱国[1] WEN Liuying;LIU Dan;GONG Min;MA Bing;SHEN Lili;LUO Chenggang;REN Min;CHENG Lirui;JIANG Caihong;YANG Aiguo(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Tobacco Gene Resources,Qingdao 266101,China)

机构地区：[1]中国农业科学院烟草研究所,烟草行业烟草基因资源利用重点实验室,青岛266101

出　　处：《中国烟草科学》2018年第4期18-25,31,共9页Chinese Tobacco Science

基　　金：中国烟草总公司重大专项项目“以K326为主要底盘品种的烟草全基因组模块构建”(110201601028JY-02);中国烟草总公司重大专项项目“烟草抗CMV和赤星病连锁分子标记开发及NC82、K326品种抗性改良”(110201301009,JY-09);中央级公益性科研院所基本科研业务费专项“CMV病毒侵染烟草叶片过程的电镜样品制备技术研究”(1610232016015)。

摘　　要：Y48是对主栽烤烟品种K326的病毒病抗性进行定向改良培育出的新品系,在保持原K326其他性状不变的前提下,对TMV和CMV抗性显著提高。为揭示其在细胞学方面的抗病机制,本研究利用透射电子显微镜(TEM)对TMV接种0~72 h后的K326和Y48叶片进行超微结构观察。结果显示,K326接种24 h时,叶绿体类囊体片层减少,线粒体膨大,胞内出现自噬结构,接种72 h时,叶绿体、线粒体被病毒完全破坏,叶肉细胞病理性坏死;而Y48,在接种48 h时才发生叶绿体内部沉积少量颗粒,线粒体嵴消失,胞内出现降解的囊泡,接种72 h叶绿体,线粒体全部降解,细胞质凝集,叶肉细胞程序化死亡。结果表明,相对于K326,Y48可以延迟TMV病毒的侵染,并启动超敏反应,发生HR-PCD,并且Y48接种8 h后出现过氧化物酶体,可能影响细胞内ROS代谢水平,二者或许是Y48对TMV表现抗病的细胞学证据,研究结果为进一步解析Y48的抗病分子机理奠定了基础。A new variety with genetic improvement of K326(Y48)has great market potential.Y48 was developed by marker-assisted selection for its high anti-TMV&CMV ability with the same agronomic traits.In order to reveal the cytological mechanism of the Y48 resistance,transmission electronic microscopy(TEM)was use to observe the ultrastructure of K326 and Y48 leaves in 0-72 h after TMV inoculation.The results showed that,in K326,chloroplast thylakoid lamellae decreased,mitochondria crista expanded,and autophagy appeared in mesophyll cells at 24 h after TMV inoculation.The chloroplasts and mitochondria were completely destroyed by TMV,then mesophyll cells went through pathological necrosis at 72 h after inoculation.While in Y48,a small amount of particles were deposited inside the chloroplast,the mitochondria cristae degraded,and the degraded-like vesicles appeared in mesophyll cells at 48 h after TMV inoculation.Chloroplasts and mitochondria were degraded totally,cytoplasm was concentrated,mesophyll cells went through programmed cell death(PCD)at 72 h after inoculation.The results indicated that,compared to K326,Y48 could delay TMV infection and activate hypersensitivity response to lead mesophyll cell to go through HR-PCD.Add,the present of peroxisome in Y48 at 72 h agter inoculation,might effect ROS level of cell.The two differences might provide cytological evidence to reveal the Y48 resistance and give the clue to analyze the molecular resistance mechanism of Y48.

关 键 词：烟草普通花叶病 透射电子显微镜 超微结构 超敏反应 细胞程序化死亡 

分 类 号：S572.03[农业科学—烟草工业]